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MEMBRANE-BASED ASSAYS USING TIME-RESOLVED FLUORESCENCE
MEMBRANE-BASED ASSAYS USING TIME-RESOLVED FLUORESCENCE
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机译:使用时间分辨荧光的基于膜的分析
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摘要
A membrane-based assay device for detecting the presence or quantity of ananalyte residing in a test sample is provided. The device utilizes time-resolved fluorescence to detect the signals generated by excited fluorescentlabels. Because the labels can have relatively long emission lifetime, short-lived background interference can be practically eliminated through delayedfluorescence detection. In addition, the resulting fluorescent reader can havea simple and inexpensive design. For instance, in one embodiment, the readercan utilize a silicon photodiodide and a pulsed light-emitting diode (LED) toaccurately excite labels and detect fluorescence on a membrane-based assaydevice without requiring the use of expensive components, such asmonochromators or narrow emission band width optical filters.
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