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MEMBRANE-BASED ASSAYS USING TIME-RESOLVED FLUORESCENCE

机译:使用时间分辨荧光的基于膜的分析

摘要

A membrane-based assay device for detecting the presence or quantity of ananalyte residing in a test sample is provided. The device utilizes time-resolved fluorescence to detect the signals generated by excited fluorescentlabels. Because the labels can have relatively long emission lifetime, short-lived background interference can be practically eliminated through delayedfluorescence detection. In addition, the resulting fluorescent reader can havea simple and inexpensive design. For instance, in one embodiment, the readercan utilize a silicon photodiodide and a pulsed light-emitting diode (LED) toaccurately excite labels and detect fluorescence on a membrane-based assaydevice without requiring the use of expensive components, such asmonochromators or narrow emission band width optical filters.
机译:基于膜的测定装置,用于检测是否存在或存在提供了存在于测试样品中的分析物。设备利用时间-分辨荧光以检测激发荧光产生的信号标签。由于标签的发射寿命较长,因此实际的背景干扰可以通过延迟来消除荧光检测。此外,所得的荧光阅读器可以具有简单而便宜的设计。例如,在一个实施例中,阅读器可以利用光电二极管硅和脉冲发光二极管(LED)在基于膜的测定中准确激发标签并检测荧光设备,而无需使用昂贵的组件,例如单色仪或窄发射带宽的光学滤光片。

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