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首页> 外文学位 >The development and application of a white-light confocal microscope in the morphologic and anatomic characterization of cells within the cornea.
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The development and application of a white-light confocal microscope in the morphologic and anatomic characterization of cells within the cornea.

机译：白光共聚焦显微镜在角膜内细胞形态和解剖学表征中的开发和应用。

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摘要

Microscopy lies at the cornerstone of contemporary histology and microanatomy. Cellular research, whether with the standard light microscope or the electron microscope, reveals valuable insights into cellular function, structure, and physiology. Unfortunately, conventional modes of microscopy only produce static, two-dimensional images of the tissue under investigation. With the white-light confocal microscope, high resolution, high magnification, dynamic images can be obtained in vivo. This study examines the development and application of a white-light confocal microscope for the characterization of cells within the normal cornea and in certain pathological states of the cornea of the eye. This instrument facilitates the correlation of cell structure and function in the most natural state.; A confocal microscope utilizing a fiber-optic light source and single-sided disc design was constructed for these studies. The normal anatomy of the cornea was characterized using the white-light confocal microscope and compared to that observed with the compound research microscope and the electron microscope. Confocal microscopy provided a dynamic, three-dimensional examination of the corneal epithelium and the subepithelial nerve plexus. The stromal keratocyte nuclei and the corneal endothelium could be observed. No stains or dyes were necessary with the white-light confocal microscope, and since the microscope is used in vivo, fixation artifacts were avoided.; Characteristics of Fuchs' dystrophy, posterior polymorphous dystrophy, contact lens related corneal changes, infectious keratitis, and ophthalmia nodosa were granular dystrophy, a new type of amyloid dystrophy, Chandler's dystrophy, Cogan's dystrophy, contact lens related corneal changes, infectious keratitis, and ophthalmia nodosa were identified in vivo utilizing the white-light confocal microscope. These studies show, for the first time, that the white-light confocal microscope can be used to characterize these pathologic conditions. This new instrument promises to be a useful tool in the field of confocal microscopy, extending studies presently carried out in tissue culture, or tissue section to in vivo conditions.
机译：显微术是当代组织学和显微解剖学的基石。无论是使用标准光学显微镜还是电子显微镜进行的细胞研究，都可以揭示对细胞功能，结构和生理学的宝贵见解。不幸的是，常规的显微镜检查模式只能产生所研究组织的静态二维图像。使用白光共聚焦显微镜，可以在体内获得高分辨率，高放大倍率的动态图像。这项研究检查了白光共聚焦显微镜的开发和应用，该显微镜用于表征正常角膜和眼角膜某些病理状态下的细胞。该仪器促进了最自然状态下细胞结构与功能的相关性。针对这些研究，构建了使用光纤光源和单面光盘设计的共聚焦显微镜。使用白光共聚焦显微镜对角膜的正常解剖结构进行了表征，并将其与复合研究显微镜和电子显微镜所观察到的进行了比较。共聚焦显微镜可对角膜上皮和上皮下神经丛进行动态三维检查。可以观察到间质角膜细胞核和角膜内皮。白光共聚焦显微镜无需染色或染料，并且由于该显微镜是在体内使用的，因此避免了固定伪影。 Fuchs营养不良，后部多态性营养不良，角膜接触镜相关角膜变化，感染性角膜炎和结节性眼球炎的特征是颗粒性营养不良，新型淀粉样营养不良，Chandler营养不良，Cogan营养不良，隐形眼镜相关角膜变化，感染性角膜炎。利用白光共聚焦显微镜在体内鉴定了结节。这些研究首次表明，白光共聚焦显微镜可用于表征这些病理状况。这种新仪器有望成为共聚焦显微镜领域的有用工具，将目前在组织培养或组织切片中进行的研究扩展到体内条件。

著录项

  • 作者

    Kaufman, Stephen C.;

  • 作者单位

    Louisiana State University Health Sciences Center.;

  • 授予单位 Louisiana State University Health Sciences Center.;
  • 学科 Biology Anatomy.; Biology Cell.; Health Sciences Ophthalmology.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 118 p.
  • 总页数 118
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物形态学;细胞生物学;
  • 关键词

  • 入库时间 2022-08-17 11:49:25

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