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首页> 外文学位 >Interaction of cellulase and xylanase with recycled bleached kraft fiber.
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Interaction of cellulase and xylanase with recycled bleached kraft fiber.

机译：纤维素酶和木聚糖酶与再生漂白牛皮纸纤维的相互作用。

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摘要

Biotechnology involving modification of recycled fiber with enzymes offers new, environmentally friendly methods of utilizing wastepaper sources. Specifically, enzymatic deinking and enzymatic drainage improvement with cellulases and xylanases offer new ways of dealing with some of the historical problems associated with recycling wastepaper, e.g., high chemical consumption and paper machine operating problems. The mode of action of the enzymes on recycled fiber, however, is not well understood.;Thus, several studies were performed to characterize the interaction of cellulases and xylanases on high quality, recycled bleached kraft fiber and, hopefully, to help identify new biotechnical approaches for enzymatic modification of recycled fiber. Simulated recycled bleached kraft fiber was studied with respect to enzyme adsorption, hydrolysis, and enzyme activity recovery. Drying dramatically decreased the kraft fiber's capacity to adsorb cellulases compared to never-dried fiber. Xylanases showed little affinity for this type of fiber substrate. High surface area material, i.e., fines, in a papermaking system is preferentially attacked by the enzymes. It was suggested that such material protects the long fiber from the enzymes' action. Depending on the fiber type in use, cellulase and xylanase activity adsorbed on the fiber during the biotechnical procedures has the potential to be recovered, either by recycling the hydrolysate or washing with dilute alkali and a non-ionic surfactant.;An additional application for xylanase was identified; the use of cold alkali extractions in combination with xylanase treatment can purify once-dried bleached kraft hardwood fiber and wastepaper sources high in bleached hardwood kraft fiber content to carbohydrate levels and viscosities similar to chemical cellulose, or dissolving pulps. Xylanase accessibility to xylan, lost during drying in the papermaking process, can be recovered by applying an initial cold alkali extraction to the fiber.
机译：涉及用酶改性再生纤维的生物技术提供了利用废纸来源的新的，环境友好的方法。具体而言，纤维素酶和木聚糖酶的酶促脱墨和酶促排水改进提供了处理与废纸再循环相关的一些历史问题（例如，高化学消耗和造纸机操作问题）的新方法。然而，酶对再生纤维的作用方式尚不十分清楚。因此，进行了一些研究来表征纤维素酶和木聚糖酶在高质量，再生漂白牛皮纸纤维上的相互作用，并希望有助于鉴定新的生物技术。再生纤维的酶促改性方法。对模拟的再生漂白牛皮纸纤维进行了酶吸附，水解和酶活性回收方面的研究。与从未干燥的纤维相比，干燥显着降低了牛皮纸纤维吸收纤维素酶的能力。木聚糖酶对这种类型的纤维底物几乎没有亲和力。造纸系统中的高表面积材料（即细粉）优先受到酶的攻击。有人提出这种材料可以保护长纤维免受酶的作用。根据使用的纤维类型，在生物技术过程中吸附在纤维上的纤维素酶和木聚糖酶活性有可能被回收，方法是通过循环利用水解产物或用稀碱和非离子表面活性剂洗涤。被确定；冷碱提取物与木聚糖酶处理的结合使用可以纯化曾经干燥过的漂白牛皮纸硬木纤维和废纸，这些废纸中的漂白硬木牛皮纸纤维含量很高，其碳水化合物含量和粘度类似于化学纤维素或溶解纸浆。在造纸过程中干燥过程中失去的木聚糖酶对木聚糖的可及性，可以通过对纤维进行初始的冷碱提取来恢复。

著录项

  • 作者

    Jackson, Larry Scott.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Engineering Chemical.;Agriculture Wood Technology.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 203 p.
  • 总页数 203
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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