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Development of transgenic plants with non-plant antibacterial protein genes for resistance to bacterial pathogens.

机译：具有非植物抗菌蛋白基因的转基因植物对细菌病原体的抗性开发。

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摘要

Recent advances in genetic engineering have made it possible to transform non-plant genes into crop plants. Some progresses have been made in the development of transgenic plants with non-plants with non-plant antibacterial protein genes for resistance to bacterial pathogens. The mammalian gene encoding human lactoferrin (HLF), an iron-binding glycoprotein, has not been used previously in developing transgenic plants for bacterial disease resistance.;In an attempt to develop transgenic plants using antibacterial protein genes to enhance bacterial disease resistance, we first developed transgenic calli using a tobacco suspension cell culture to express HLF. Transgenic calli produced truncated HLF which were much smaller (about 48 kDa) than full length HLF (80 kDa). Total protein extracts made from transgenic calli containing the truncated HLF exhibited much higher antibacterial effects than calli containing the full length HLF on four representative phytopathogenic bacteria. Consequently, transgenic tobacco (Nicotiana tabacum) plants expressing the human lactoferrin cDNA were further developed using Agrobacterium tumefaciens strain A2760. All T;In order to introduce a lactoferrin gene into Phaseolus vulgaris, factors influencing both Agrobacterium-, or biolistic-mediated transformation of this plant were studied using a GUS intron containing construct. Factors significantly affecting Agrobacterium transformation included preculture and cocultivation conditions, explant age and maturity, host-range, and selection pressure. Using optimized transformation conditions, about 4% of total shoots or buds were putatively transformed. Parameters influencing biolistic transformation of P. vulgaris were also optimized, based on embryonated cotyledons as target tissues.
机译：基因工程的最新进展使得将非植物基因转化为农作物成为可能。在具有非植物的非植物抗细菌病原体基因的非植物转基因植物的开发中已经取得了一些进展。编码人类乳铁蛋白（HLF）（一种铁结合糖蛋白）的哺乳动物基因以前尚未用于开发具有细菌抗病性的转基因植物。为了尝试使用抗菌蛋白基因增强细菌抗病性来开发转基因植物，我们首先利用烟草悬浮细胞培养技术开发了转基因愈伤组织来表达HLF。转基因愈伤组织产生的截短的HLF比全长HLF（80 kDa）小得多（约48 kDa）。由含有截短的HLF的转基因愈伤组织制成的总蛋白提取物比含有全长HLF的愈伤组织对四种代表性植物病原菌具有更高的抗菌作用。因此，使用根癌土壤杆菌菌株A2760进一步表达人乳铁蛋白cDNA的转基因烟草（Nicotiana tabacum）植物。为了将乳铁蛋白基因引入寻常菜豆，使用含有GUS内含子的构建体研究了影响农杆菌或生物弹介导的该植物转化的因素。显着影响农杆菌转化的因素包括预培养和共培养条件，外植体年龄和成熟度，寄主范围和选择压力。使用优化的转化条件，推定转化了约4％的新芽或新芽。基于胚子叶作为靶组织，还优化了影响寻常型毕赤酵母生物转化的参数。

著录项

  • 作者

    Zhang, Zhanyuan.;

  • 作者单位

    The University of Nebraska - Lincoln.;

  • 授予单位 The University of Nebraska - Lincoln.;
  • 学科 Agriculture Plant Pathology.;Biology Molecular.;Biology Microbiology.;Biology Genetics.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 185 p.
  • 总页数 185
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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