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首页> 外文学位 >Structural insights into the function of the Arabidopsis protein RIN4, a multi-regulator of plant resistance against bacterial pathogens.
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Structural insights into the function of the Arabidopsis protein RIN4, a multi-regulator of plant resistance against bacterial pathogens.

机译：拟南芥蛋白RIN4（一种针对植物对细菌病原体的抗性的多重调节剂）的功能的结构见解。

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摘要

The plant innate immune system consists of two main branches, PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI). Perception of pathogen-associated molecular patterns (PAMPs) activates PTI. However, pathogenic bacteria deploy virulence factors that suppress PTI, including translocated type III effector (T3E) proteins. In addition to suppressing PTI, T3Es also activate the second branch of plant innate immunity, ETI, via activation of NB-LRR disease resistance proteins. ETI can be indirectly activated by PTI-suppressing T3Es. For instance, the Arabidopsis protein RIN4, which negatively regulates PTI, is targeted by the three sequence unrelated T3Es AvrRpm1, AvrB, and AvrRpt2 from P. syringae that suppress PTI. Additionally, RIN4 mediates recognition of these T3Es via the NB-LRR proteins RPM1 and RPS2. AvrRpm1 and AvrB phosphorylate RIN4, and AvrRpt2 cleaves it. RIN4 interacts in planta with, RPM1 and RPS2, and perturbation of RIN4 elicits their response to these T3Es. Structure-function analysis of RIN4 has revealed that (1) RIN4 has multiple domains capable of negatively regulating PTI; (2) one of these domains is critical for regulation of RPM1; (3) membrane targeting of RIN4 differentially affects its ability to regulate PTI and ETI; and (4) RIN4 differentially regulates RPM1 and RPS2. Collectively, our results indicate that RIN4 is multifunctional with separable activities involved in regulation of these two central branches of plant immunity.
机译：植物的先天免疫系统由两个主要分支组成，PAMP触发的免疫（PTI）和效应子触发的免疫（ETI）。病原体相关分子模式（PAMPs）的感知激活PTI。但是，致病细菌会部署抑制PTI的毒力因子，包括易位的III型效应子（T3E）蛋白。除了抑制PTI，T3E还通过激活NB-LRR抗病蛋白来激活植物固有免疫力的第二个分支ETI。 ETI可以通过抑制PTI的T3E间接激活。例如，负调控PTI的拟南芥蛋白RIN4被来自丁香假单胞菌的三个序列无关的T3Es AvrRpm1，AvrB和AvrRpt2靶向，从而抑制了PTI。此外，RIN4通过NB-LRR蛋白RPM1和RPS2介导这些T3E的识别。 AvrRpm1和AvrB磷酸化RIN4，AvrRpt2裂解它。 RIN4在植物中与RPM1和RPS2相互作用，并且RIN4的扰动引起它们对这些T3E的反应。 RIN4的结构功能分析表明：（1）RIN4具有多个能够负调控PTI的结构域； （2）这些域之一对RPM1的调节至关重要； （3）RIN4的膜靶向作用会不同地影响其调节PTI和ETI的能力； （4）RIN4差分调节RPM1和RPS2。总体而言，我们的结果表明RIN4具有多功能性，具有参与调节植物免疫力这两个主要分支的活动。

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  • 作者

    da Cunha, Luis C. V.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Agriculture Horticulture.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 212 p.
  • 总页数 212
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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