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首页> 外文学位 >Molecular cytogenetic genome analysis of a cultivated cotton (Gossypium hirsutum L.) and its putative diploid ancestors.
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Molecular cytogenetic genome analysis of a cultivated cotton (Gossypium hirsutum L.) and its putative diploid ancestors.

机译：栽培棉花（棉（Gossypium hirsutum L.）及其假定的二倍体祖先）的分子细胞遗传基因组分析。

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摘要

Fluorescent in situ hybridization (FISH) was performed on metaphase chromosomes of the cotton AD-genome tetraploid Gossypium hirsutum and diploid species closely related to its ancestors. Four classes of probes were used: a bacterial artificial chromosome (BAC), 5S and 18S-28S rDNA repeats, a Tyl-copia-like retrotransposon and 20 uncharacterized interspersed repetitive elements. BAC-FISH demonstrated the reliable detection of single/low-copy genomic clones in plants, establishing a powerful tool for physical mapping. Eleven 18S-28S rDNA loci were detected in G. hirsutum; 5 in the A-subgenome and 6 in the D-subgenome. The G. hirsutum subgenomes each had 2 more 18S-28S rDNA loci than putative A-genome and D-genome diploid ancestors of the tetraploid. Dual-color FISH of 5S and 18S-28S rDNA loci allowed size comparisons between rDNA loci on homoeologous chromosomes, revealing significant changes in the sizes of Gossypium 18S-28S rDNA loci. Tyl-copia like retrotransposon clone A108 yielded strong FISH signal on all G. hirsutum chromosomes, roughly equal in intensity between the A- and D-subgenomes. Hybridization of A108 to G. arboreum gave similar results, whereas no signal was detected on chromosomes of the D-genome diploid G. raimondii, the putative diploid ancestor of the G. hirsutum D-subgenome. FISH of 20 G. hirsutum repetitive elements revealed that 17 of the 20 occurred in both subgenomes of G. hirsutum, while 3 were specific to the A-subgenome. Eight elements, 2 subgenome-specific and 6 non-subgenome specific elements, were selected for FISH to G. arboreum (A
机译：在棉花AD基因组四倍体陆地棉和与其祖先密切相关的二倍体物种的中期染色体上进行了荧光原位杂交（FISH）。使用了四类探针：细菌人工染色体（BAC），5S和18S-28S rDNA重复序列，Tyl-copia样逆转录转座子和20个未表征的散布的重复元件。 BAC-FISH证明了对植物中单拷贝/低拷贝基因组克隆的可靠检测，建立了强大的物理作图工具。在陆地棉中检测到11个18S-28S rDNA基因座。 A-亚基因组中有5个，D-亚基因组中有6个。与四倍体的推定A基因组和D基因组二倍体祖先相比，G。hirsutum亚基因组各具有2个更多的18S-28S rDNA基因座。 5S和18S-28S rDNA基因座的双色FISH可以比较同源染色体上rDNA基因座的大小，揭示了棉18S-28S rDNA基因座大小的显着变化。 Tyl-copia样反转录转座子克隆A108在所有G.hirsutum染色体上产生强FISH信号，强度在A和D亚基因组之间大致相等。 A108与G. arboreum的杂交得到了相似的结果，而在D-基因组二倍体雷蒙德氏菌（R. hirsutum D-亚基因组的假定二倍体祖先）的染色体上未检测到信号。 FISH的20个G. hirsutum重复元件揭示了20个中的17个在G. hirsutum的两个亚基因组中发生，而3个对A-亚基因组具有特异性。选择了8个元素，即2个亚基因组特异性元素和6个非亚基因组特异性元素，用于FISH至G.arboreum（A

著录项

  • 作者

    Hanson, Robert Eldon, Jr.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Agriculture Agronomy.;Biology Genetics.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 97 p.
  • 总页数 97
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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