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首页> 外文学位 >Genetic analysis of wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus strain BD413.
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Genetic analysis of wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus strain BD413.

机译：钙不动杆菌BD413菌株蜡酯和三酰基甘油生物合成的遗传分析。

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摘要

The phenomena I have investigated is the accumulation of wax esters and triacylglycerol in the form of intracellular inclusions in the gram negative aerobic bacterium Acinetobacter calcoaceticus strain BD413. This strain of A. calcoaceticus accumulates both wax esters and triacylglycerol as a means of carbon storage when it undergoes nutrient starvation.;Nutritional supplementation experiments on the wax;By expressing the identified open reading frame from A. calcoaceticus in E. coli, and assaying for the encoded protein's activity, the genes enzymatic activity was identified. The gene product was observed to catalyze the formation of fatty alcohol from acyl-CoA via a fatty aldehyde intermediate. The isolated gene has been named acr1 for acyl-CoA reductase.;A second aspect of the research described herein concerns mutants isolated following transposon mutagenesis with mini-Tn10PttKm. Flanking sequence surrounding a transposon insertion in strain 11-C7, a wax;Mutants of strain BD413 were induced using both chemical and transposon mutagenesis methods. By screening colonies for wax accumulation by staining with the lipophilic dye, Sudan black B, followed by TLC, a total of 8 transposon mutants and 21 chemically induced mutants were isolated. These mutants were separated into 3 general categories: wax
机译：我研究的现象是革兰氏阴性需氧细菌钙乙酸不动杆菌菌株BD413中细胞内夹杂物形式的蜡酯和三酰基甘油的积累。这种钙曲霉菌株在营养缺乏时会同时积累蜡酯和三酰基甘油，以作为碳储存的手段。对蜡的营养补充实验；通过在大肠杆菌中表达已鉴定的钙曲霉开放阅读框，并进行测定对于编码蛋白的活性，鉴定了基因的酶促活性。观察到该基因产物经由脂肪醛中间体催化从酰基辅酶A形成脂肪醇。该分离的基因已被命名为酰基-CoA还原酶的acr1。本文所述研究的第二方面涉及在用mini-Tn10PttKm进行转座子诱变后分离的突变体。在蜡11-C7菌株中围绕转座子插入的侧翼序列；使用化学和转座子诱变方法诱导菌株BD413的突变。通过用亲脂性染料苏丹黑B染色，然后进行TLC染色，筛选菌落中蜡的积累，共分离出8个转座子突变体和21个化学诱导的突变体。这些突变体分为3大类：蜡

著录项

  • 作者

    Reiser, Steven Edward.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Chemistry Biochemistry.;Biology Microbiology.;Biology Molecular.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 194 p.
  • 总页数 194
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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