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首页> 外文学位 >Development of plant disease suppressive compost-amended biocontrol agent-fortified potting mixes and turf topdressings and use of random amplified polymorphic DNA markers for identification of Trichoderma hamatum 382.
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Development of plant disease suppressive compost-amended biocontrol agent-fortified potting mixes and turf topdressings and use of random amplified polymorphic DNA markers for identification of Trichoderma hamatum 382.

机译：开发了植物病害抑制堆肥改良的生物防治剂，增强了盆栽混合物和草坪追肥，并使用了随机扩增的多态性DNA标记物来鉴定木霉382。

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摘要

The objectives of this project were to: (1) develop a commercial-scale process for the production of biocontrol agent-fortified compost-amended potting mixes consistently suppressive to several soilborne plant pathogens; (2) assess biocontrol agent-fortified compost topdressings for efficacy in control of dollar spot (Sclerotinia homoeocarpa) on creeping bentgrass cv. "Penncross"; and (3) generate a random amplified polymorphic DNA (RAPD) system for detection and identification of the introduced but indigenous biocontrol agent Trichoderma hamatum 382.;Potting mixes, prepared with various batches of composted softwood bark and/or yard trimmings fortified with the biocontrol agents Trichoderma hamatum 382 (Th382) and Flavobacterium balustinum 299;Efficacy of composted municipal biosolids topdressings fortified with the biocontrol agents Th382 and ;Random amplified polymorphic DNA (RAPD) banding patterns were generated for 65 isolates of T. hamatum, T. harzianum, T. koningii, T. viride and Gliocladium virens, including the biocontrol agent Th382, utilizing a single primer polymerase chain reaction (PCR) assay. Unique RAPD patterns generated with ten random primers differentiated Th382 from other closely related fungi. RAPD patterns were highly consistent for single-spore isolates of Th382. Cluster analysis (centroid method) based on Dice similarity coefficients, revealed great diversity in patterns of isolates of G. virens and Trichoderma spp. including isolates of T. hamatum.
机译：该项目的目标是：（1）开发一种商业规模的方法，用于生产始终抑制多种土壤传播植物病原体的生物防治剂强化堆肥改良盆栽混合物； （2）评估生物防治剂强化的堆肥追肥对防治bent草Cv上美元斑（Sclerotinia homoeocarpa）的功效。 “佩恩克罗斯”； （3）生成随机扩增的多态DNA（RAPD）系统，用于检测和鉴定引入的但本土的生物防治剂木霉Trichoderma hamatum 382。细菌木霉382（Th382）和牛黄杆菌299;用生物防治剂Th382和强化的堆肥市政生物固体追肥的功效;为65个分离的海生丁香（T. hamatum），哈茨木霉（T. harzianum），丁香（T. hamatum）生成了随机扩增多态性DNA（RAPD）条带模式。利用单引物聚合酶链式反应（PCR）测定法，包括生物防治剂Th382的康宁氏单胞菌，绿头孢霉和毒状胶体。用十种随机引物产生的独特RAPD模式将Th382与其他紧密相关的真菌区分开来。对于Th382的单孢子分离株，RAPD模式高度一致。基于Dice相似系数的聚类分析（质心法）显示，G。virens和木霉属分离株的模式差异很大。包括沙门氏菌的分离株。

著录项

  • 作者

    Grebus, Marcella Elaine.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Agriculture Plant Pathology.;Agriculture Agronomy.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 1995
  • 页码 152 p.
  • 总页数 152
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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