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首页> 外文学位 >Part~1. A dual ion source to produce cesium(+) and iodine(-) ions for secondary ion mass spectrometry. Part~2. Investigations of matrix-assisted laser desorption with a reflecting time-of-flight mass spectrometer.
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Part~1. A dual ion source to produce cesium(+) and iodine(-) ions for secondary ion mass spectrometry. Part~2. Investigations of matrix-assisted laser desorption with a reflecting time-of-flight mass spectrometer.

机译：部分〜1。产生铯（+）和碘（-）离子的双离子源，用于二次离子质谱分析。第二部分用反射飞行时间质谱仪研究基质辅助激光解吸。

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摘要

dual Cs;The reflecting time-of-flight mass spectrometer (Manitoba TOF II) has been modified for matrix-assisted laser desorption (MALD) mass spectrometry. The performance of the MALD TOF mass spectrometer has been examined, including sensitivity, mass range and resolution. The MALD mass spectrometric analysis of IcIR protein, including its molecular weight and the verification of its sequence, is particularly described.;Measurements of kinetic energy distributions of molecular ions ejected into an electric field by matrix-assisted laser desorption were performed in a reflecting time-of-flight mass spectrometer by measuring the reflected molecular ion signal as a function of the difference between the accelerating voltage and mirror voltage. The molecular ions were found to have less kinetic energy than expected from the accelerating potential, i.e., an energy deficit was observed. Under conditions typical for a matrix-assisted laser desorption experiment, the deficit is about 24 eV for positive molecular ions of insulin. The energy deficit increases with the intensity of molecular ion signal, and the molecular weight of the protein; it is also larger for negative molecular ions than for positive molecular ions of insulin. Singly and doubly charged positive molecular ions have similar energy deficits.;The metastable decay of insulin produced by matrix-assisted laser desorption has been examined in a reflecting time-of-flight mass spectrometer. The (M-H)
机译：双Cs;反射飞行时间质谱仪（Manitoba TOF II）已经过改进，可用于基质辅助激光解吸（MALD）质谱。已检查了MALD TOF质谱仪的性能，包括灵敏度，质量范围和分辨率。特别描述了IcIR蛋白的MALD质谱分析，包括其分子量和序列验证。;在反射时间内对通过基质辅助激光解吸喷射到电场中的分子离子的动能分布进行了测量。飞行质谱仪，通过测量反射的分子离子信号作为加速电压和反射镜电压之间差的函数。发现分子离子的动能小于加速电位所预期的动能，即观察到能量不足。在典型的基质辅助激光解吸实验条件下，胰岛素的正分子离子的亏空约为24 eV。能量赤字随分子离子信号强度和蛋白质分子量的增加而增加。对于负分子离子来说，它也比胰岛素的正分子离子更大。单电荷和双电荷的正分子离子具有相似的能量亏空。在反射飞行时间质谱仪中检查了由基质辅助激光解吸产生的胰岛素的亚稳态衰变。 （M-H）

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  • 作者

    Zhou, Jie.;

  • 作者单位

    University of Manitoba (Canada).;

  • 授予单位 University of Manitoba (Canada).;
  • 学科 Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 1994
  • 页码 194 p.
  • 总页数 194
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
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