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首页> 外文学位 >Modulation of plant gene expression using antisense technology and gene amplification: Alteration of shikimate pathway gene expression.
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Modulation of plant gene expression using antisense technology and gene amplification: Alteration of shikimate pathway gene expression.

机译：使用反义技术和基因扩增调节植物基因表达：sh草酸酯途径基因表达的改变。

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摘要

he effect of genetic alterations on carbon flow through the shikimate pathway, using gene amplification or antisense RNA expression, was examined. Transgenic potato plants containing a chimeric 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS) antisense gene were produced to examine the role(s) of DAHPS in plant development. Wound-induced DAHPS gene expression (enzyme activity, protein, and mRNA) was reduced in wounded tuber tissue of three independent transgenic plants expressing DAHPS antisense RNA. DAHPS mRNA levels were reduced in shoot tips and/or stem tissue of five antisense plants. These plants had reduced stem diameter and/or stem lignification compared to untransformed plants. These alterations may in part be due to reduced carbon flow through the shikimate pathway.;Gene amplification allows an organism to increase the expression of a gene(s), particularly in response to environmental stress. The stability and expression of amplified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) genes was examined in glyphosate resistant tobacco cells and regenerated plantlets. Resistant cells grown without glyphosate for over 3 years stably maintained high levels of EPSPS gene amplification; however, resistance to glyphosate declined 7-fold compared to cells continously grown on glyphosate. Similarly, plantlets regenerated from resistant cells retained levels of EPSPS gene amplification comparable to that in resistant cells, but resistance to glyphosate was greatly reduced. EPSPS mRNA levels in plantlets were reduced relative to those in resistant cells. Regenerated plantlets were altered morphologically; this was correlated with an increased genome size.;The potential of utilizing gene amplification to increase the expression of nonselectable genes was examined. A transgenic petunia cell culture containing neomycin phosphotransferase II (npt II) and
机译：使用基因扩增或反义RNA表达，研究了遗传改变对sh草酸途径碳流动的影响。产生了含有嵌合的3-脱氧-D-阿拉伯糖-庚酸七磷酸合酶（DAHPS）反义基因的转基因马铃薯植株，以检验DAHPS在植物发育中的作用。伤口诱导的DAHPS基因表达（酶活性，蛋白质和mRNA）在表达DAHPS反义RNA的三种独立转基因植物的块茎组织中降低。五种反义植物的茎尖和/或茎组织中的DAHPS mRNA水平降低。与未转化的植物相比，这些植物的茎直径和/或茎木质化程度降低。这些改变可能部分是由于通过the草酸酯途径的碳流量减少所致。基因扩增使生物体能够增加基因的表达，特别是对环境压力的响应。在草甘膦抗性烟草细胞和再生小植株中检测了5-烯醇丙酮酸ru草酸酯-3-磷酸合酶（EPSPS）基因扩增的稳定性和表达。在没有草甘膦的情况下生长超过3年的抗性细胞稳定地维持了高水平的EPSPS基因扩增；然而，与在草甘膦上连续生长的细胞相比，对草甘膦的抗性下降了7倍。同样，从抗性细胞再生的小植株保留了与抗性细胞相当的EPSPS基因扩增水平，但对草甘膦的抗性大大降低。相对于抗性细胞，小植株中EPSPS mRNA水平降低。再生的苗在形态上发生改变；这与基因组大小的增加有关。研究了利用基因扩增增加非选择基因表达的潜力。转基因矮牵牛细胞培养物中含有新霉素磷酸转移酶II（npt II）和

著录项

  • 作者

    Jones, James Dale.;

  • 作者单位

    Purdue University.;

  • 授予单位 Purdue University.;
  • 学科 Plant biology.;Biology.;Molecular biology.
  • 学位 Ph.D.
  • 年度 1994
  • 页码 171 p.
  • 总页数 171
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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