• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Modification of formed-in-place microfiltration membranes for specific protein separations and fundamental studies of affinity membrane separation processes and protein-ligand interactions.
【24h】

Modification of formed-in-place microfiltration membranes for specific protein separations and fundamental studies of affinity membrane separation processes and protein-ligand interactions.

机译：用于特定蛋白质分离的原位微滤膜的修饰以及亲和膜分离过程和蛋白质-配体相互作用的基础研究。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Formed-In-Place (FIP) titania microfiltration membranes were coated with poly (ethylene imine) (PEI) and subsequently crosslinked with multifunctional glutaraldehyde and 1,4-butanediol diglycidyl ether to a stable layer. These membranes were first studied as anion exchange membranes for protein separations using sequential separation procedures. The effects of operating parameters, pH and concentrations of protein solutions on binding and purification properties were determined. In order to achieve more specific protein separations, an affinity ligand Cibacron Blue F3GA (CBF3GA) was immobilized on the PEI layers. The separation properties of the affinity membranes were investigated with human serum albumin (HSA) solutions, including the effects of pH and ionic strength of the protein solution on the binding capacity of the membrane and the influence of the crossflow velocity on the frontal elution profile.; A mathematical model was developed to analyze the frontal performance of the affinity membranes for the protein separations. Predictions by the mathematical model using equilibrium batch adsorption data dissociation constant, K{dollar}sb{lcub}rm d{rcub}{dollar}, and maximum amount adsorbed, q{dollar}sb{lcub}rm m{rcub}{dollar}, and characteristics of the membrane for the breakthrough of HSA were described.; The interaction mechanisms between the albumins and Cibacron Blue F3GA and its immobilized form, Blue Dextran, in aqueous solution was studied by spectrophotometric titration. The results showed that the association of CBF3GA to HSA is not influenced by the attachment of the dye to dextran. However, the binding of the dye molecule to dextran chain apparently hindered the accessibility of the dye to the highest affinity bind sites on the bovine serum albumin (BSA). BSA interacts with free dye with much higher affinity than with immobilized dye. The competitive titration of the dye-albumin complex with bilirubin and potassium palmitate revealed that the bilirubin, in contrast to the results of Leatherbarrow and Dean (44), did not compete with the dye for the same binding sites on both HSA and BSA. However, palmitate significantly dissociates the binding between the dye and BSA but did not appreciably affect the interaction between the dye and HSA.
机译：用聚（乙烯亚胺）（PEI）覆盖就地形成（FIP）的二氧化钛微滤膜，然后用多功能戊二醛和1,4-丁二醇二缩水甘油醚交联至稳定层。这些膜首先被研究为使用顺序分离程序进行蛋白质分离的阴离子交换膜。确定了操作参数，pH和蛋白质溶液浓度对结合和纯化特性的影响。为了实现更具体的蛋白质分离，将亲和配体Cibacron Blue F3GA（CBF3GA）固定在PEI层上。用人血清白蛋白（HSA）溶液研究了亲和膜的分离特性，包括pH和蛋白质溶液的离子强度对膜结合能力的影响以及错流速度对额叶洗脱曲线的影响。 ;建立了数学模型来分析亲和膜对蛋白质分离的前沿性能。使用平衡批次吸附数据解离常数K {dollar} sb {lcub} rm d {rcub} {dollar}和最大吸附量q {dollar} sb {lcub} rm m {rcub} {dollar的数学模型进行预测}，并描述了用于穿透HSA的膜的特性。通过分光光度滴定法研究了白蛋白与Cibacron Blue F3GA及其固定形式Blue Dextran在水溶液中的相互作用机理。结果表明，CBF3GA与HSA的缔合不受染料与葡聚糖附着的影响。但是，染料分子与葡聚糖链的结合显然阻碍了染料与牛血清白蛋白（BSA）上最高亲和力结合位点的可及性。 BSA与游离染料的相互作用比与固定染料的相互作用要高得多。染料-白蛋白复合物与胆红素和棕榈酸钾的竞争性滴定表明，与Leatherbarrow和Dean（44）的结果相反，胆红素在HSA和BSA上的相同结合位点上不与染料竞争。然而，棕榈酸酯显着地解离了染料和BSA之间的结合，但是并没有明显影响染料和HSA之间的相互作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号