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首页> 外文学位 >Transgenic rice plants containing a chlorsulfuron resistance gene, a hygromycin resistance gene and the maizeactivator transposable element.
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Transgenic rice plants containing a chlorsulfuron resistance gene, a hygromycin resistance gene and the maizeactivator transposable element.

机译：含有氯磺隆抗性基因，潮霉素抗性基因和玉米激活子转座因子的转基因水稻植物。

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摘要

A mutant Arabidopsis acetolactate synthase (ALS) gene csr-1-1, conferring chlorsulfuron resistance, was tested in transgenic rice to develop an alternative selectable marker for rice transformation. Up to a 200-fold increase in chlorsulfuron resistance was detected in transgenic rice calli expressing the mutant ALS gene under control of the CaMV 35S promoter. Efficient selection of resistant protoplast colonies was achieved by using 10 to 50 nM of chlorsulfuron after transformation with the 35S/ALS gene. Fertile transgenic rice plants were successfully recovered from 35S/ALS-containing transformants. These results demonstrated that the 35S/ALS gene could be used as a selectable marker gene in rice transformation.;Progeny of protoplast-derived nontransformed rice plants and transgenic rice plants containing the hygromycin phosphotransferase (hph) gene and the maize activator (Ac) transposable element were investigated in a field experiment. Comparison with seed-derived plants for six major agronomic traits indicated that nontransformed protoplast-derived plants were essentially indistinguishable from the parent cultivar, suggesting that somaclonal variation in these plants was not significant. Hph-containing transgenic plants were relatively normal. The generally low fertility in these plants may have been caused by the hygromycin selection procedure. In the majority of transgenic rice plants, the hph gene was inherited as a single dominant locus. Transferred DNA was maintained stably in the rice genome through the third generation of selfing. Ac-containing transgenic plants produced a significantly higher frequency of phenotypic variations than protoplast-derived nontransformed plants and transgenic plants containing the hph gene. In some varied traits such as sterility and albinism were segregated as a single locus among transgenic progeny and may have been associated with the presence of an active Ac element. This field experiment enabled us to assess the traits of transgenic rice plants for transposon tagging and obtain useful mutant materials.
机译：在转基因水稻中测试了突变的拟南芥乙酰乳酸合酶（ALS）基因csr-1-1，该基因具有氯磺隆抗性，以开发水稻转化的替代选择标记。在表达突变的ALS基因的转基因水稻愈伤组织中，在CaMV 35S启动子的控制下，检测到的氯磺隆抗性提高了200倍。在用35S / ALS基因转化后，通过使用10至50 nM的氯磺隆，可以有效选择具有抗性的原生质体菌落。从含35S / ALS的转化体中成功回收了可育的转基因水稻植物。这些结果证明35S / ALS基因可用作水稻转化的选择标记基因。原生质体衍生的未转化水稻植物和含有潮霉素磷酸转移酶（hph）基因和可转座玉米激活剂（Ac）的转基因水稻植物的后代元素在野外实验中进行了研究。与来自种子的植物的六个主要农艺性状的比较表明，未转化的原生质体来源的植物与亲本品种基本没有区别，这表明这些植物的体细胞克隆变异并不显着。含Hph的转基因植物相对正常。这些植物中通常较低的繁殖力可能是由于潮霉素选择程序引起的。在大多数转基因水稻植物中，hph基因作为单个显性位点遗传。通过第三代自交将转移的DNA稳定地保持在水稻基因组中。含Ac的转基因植物产生的表型变异频率明显高于原生质体的未转化植物和含有hph基因的转基因植物。在某些不同的性状中，如不育和白化病，在转基因后代中被作为一个基因位点隔离，并且可能与活性Ac元素的存在有关。该田间实验使我们能够评估转基因水稻植株的性状以进行转座子标记并获得有用的突变材料。

著录项

  • 作者

    Li, Zhijian.;

  • 作者单位

    Louisiana State University and Agricultural & Mechanical College.;

  • 授予单位 Louisiana State University and Agricultural & Mechanical College.;
  • 学科 Agronomy.;Plant biology.
  • 学位 Ph.D.
  • 年度 1992
  • 页码 88 p.
  • 总页数 88
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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