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Towards the design of highly selective molecularly imprinted hydrogels for biosensor applications.

机译：致力于生物传感器应用的高选择性分子印迹水凝胶的设计。

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摘要

A method for the rational design of biomimetic sensors based on molecularly imprinted polymer for the detection of hydrocortisone is described and applied. The formation of adduct between hydrocortisone and the functional monomer, methacrylic acid (MAA) was confirmed using nuclear magnetic resonance (NMR) spectroscopy, which suggested creation of MAA-Hydrocortisone at the pre-polymeric mixture. In order to evaluate synthesis conditions, in situ free radical copolymerization was monitored by ATR-FTIR spectroscopy with methacrylic acid (MAA) as the functional monomer and tetra(ethylene glycol) dimethacrylate (TEGDMA) as the crosslinking agent in different solvents. The combined set of analysis allowed a better understanding of the recognition events. In addition, hydrocortisone imprinted polymers were synthesized in aqueous media. The feasibility of hydrogel-based MIPs was evaluated by measuring equilibrium swelling, template permeation, binding and electrochemical studies. The electrochemical results of 17:1 MIP demonstrated higher sensitivity toward hydrocortisone in comparison to non-MIP. Overall, this work reported the evaluation of the principal factors affecting the stabilization of functional monomer-template complex before and during the imprinting process, which provides essential information for the rational design of molecularly imprinted polymers.
机译：描述并应用了一种基于分子印迹聚合物的仿生传感器的合理设计方法，以检测氢化可的松。使用核磁共振（NMR）光谱确认了氢化可的松与功能性单体甲基丙烯酸（MAA）之间的加合物形成，这表明在预聚物混合物中生成了MAA-氢化可的松。为了评估合成条件，通过ATR-FTIR光谱法在不同溶剂中以甲基丙烯酸（MAA）作为功能单体和四（乙二醇）二甲基丙烯酸酯（TEGDMA）作为交联剂对自由基聚合进行了监测。组合的分析允许更好地理解识别事件。另外，在水介质中合成了氢化可的松印迹聚合物。通过测量平衡溶胀，模板渗透，结合和电化学研究，评估了基于水凝胶的MIP的可行性。 17：1 MIP的电化学结果表明，与非MIP相比，对氢化可的松的敏感性更高。总的来说，这项工作报告了在压印过程之前和期间影响功能性单体-模板配合物稳定化的主要因素的评估，这为分子印迹聚合物的合理设计提供了重要信息。

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  • 作者

    Padro Cortes, Lorena R.;

  • 作者单位

    University of Puerto Rico, Mayaguez (Puerto Rico).;

  • 授予单位 University of Puerto Rico, Mayaguez (Puerto Rico).;
  • 学科 Engineering Chemical.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 271 p.
  • 总页数 271
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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