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Microbiological and immunological investigation of periapical pathosis.

机译：根尖周病变的微生物学和免疫学研究。

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摘要

Infection of the root canal system of a tooth results in pathological changes consistent with both non-specific inflammation and specific immunological reactions. In this investigation, fifty strains of microorganisms were isolated from the apical 5-6 mm of ten teeth with cariously exposed pulp chambers. A mouse model was developed to macroscopically evaluate the pathogenic effects of oral microorganisms associated with endodontic disease. Explant cultures of periapical lesions were cultured with tritiated amino acids in enriched RPMI-1640 medium. Staph Protein A affinity columns were used to purify the labeled IgG in the supernatant fluids. The in vitro biosynthesis of IgG was demonstrated by detecting a significant difference in scintillation counts between the purified Staph Protein A eluent (IgG) and cocktail blanks. A dot enzyme-linked immunosorbent assay (Dot-ELISA) was used to determine the reactivity of IgG in the supernatant fluids of explant cultures of periapical lesions with a panel of ten microorganisms implicated in infections of endodontic origin. When 90 supernatant fluids were tested, black-pigmented Bacteroides/Porphyromonas (BPBP) were the microoganisms most often reacting by a Dot-ELISA (B. intermedius-84%, P. endodontalis-58%, and P. gingivalis-50%). A lack of cross reactivity with other BPBP was demonstrated by adsorbing each of four supernatant fluids with each of four BPBP and then testing for reactivity by a Dot-ELISA. A radial immunodiffusion assay was used to detect and quantitate the amount of IgG, IgA, and IgM in the supernatant fluids of explant cultures of periapical lesions. IgG was the predominant immunoglobulin followed by IgA but, IgM was not detected. A radioimmunosorbent test was used to demonstrate IgE in supernatant fluids from explant cultures of periapical lesions. An ELISA was used to determine the difference in reactivity of serum IgG to a panel of ten oral anaerobic microorganisms implicated in infections of endodontic origin. B. intermedius was associated with both endodontic and periodontal disease while P. gingivalis was associated with only periodontal disease. The results suggest that BPBP are important in polymicrobial infections of endodontic origin.
机译：牙齿的根管系统感染会导致与非特异性炎症和特异性免疫反应一致的病理变化。在这项研究中，从十颗牙齿的根尖5-6 mm的牙髓腔中暴露出了五十种微生物。开发了小鼠模型以宏观评估与牙髓病相关的口腔微生物的致病作用。在富含RPMI-1640的培养基中，用with化的氨基酸培养根尖周病变的外植体培养物。金黄色葡萄球菌蛋白A亲和柱用于纯化上清液中的标记的IgG。 IgG的体外生物合成通过检测纯化的Staph蛋白A洗脱液（IgG）和鸡尾酒空白之间的闪烁计数有显着差异来证明。使用点酶联免疫吸附试验（Dot-ELISA）来确定根尖周病变的外植体培养物上清液中IgG与一组涉及牙髓起源感染的十种微生物的反应性。当测试90种上清液时，黑色色素的拟杆菌/卟啉单胞菌（BPBP）是最常通过Dot-ELISA反应的微生物（B. intermedius-84％，P.enddontalis-58％和P. gingivalis-50％） 。通过用四种BPBP吸附每种四种上清液，然后通过Dot-ELISA测试反应性，证明与其他BPBP缺乏交叉反应性。使用径向免疫扩散测定法检测和定量根尖周病变的外植体培养物上清液中IgG，IgA和IgM的量。 IgG是主要的免疫球蛋白，其次是IgA，但未检测到IgM。放射免疫吸附试验用于证实根尖周病变的外植体培养物中上清液中的IgE。 ELISA用于确定血清IgG与一组十种涉及牙髓来源感染的口腔厌氧微生物的反应性差异。中间芽孢杆菌与牙髓疾病和牙周疾病有关，而牙龈卟啉单胞菌仅与牙周疾病有关。结果表明，BPBP在牙髓来源的微生物感染中很重要。

著录项

  • 作者

    Baumgartner, John Craig.;

  • 作者单位

    University of Maryland, Baltimore.;

  • 授予单位 University of Maryland, Baltimore.;
  • 学科 Biology Microbiology.; Health Sciences Dentistry.
  • 学位 Ph.D.
  • 年度 1990
  • 页码 263 p.
  • 总页数 263
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;口腔科学;
  • 关键词

  • 入库时间 2022-08-17 11:50:36

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