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首页> 外文学位 >PROPERTIES OF IDENTIFIED CORTICOCOLLICULAR NEURONS IN PRIMARY CULTURES OF RAT VISUAL CORTEX.
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PROPERTIES OF IDENTIFIED CORTICOCOLLICULAR NEURONS IN PRIMARY CULTURES OF RAT VISUAL CORTEX.

机译：大鼠视觉皮层原代培养中已鉴定的糖皮质神经元的性质。

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摘要

The properties of neurons from the visual cortex of postnatal Long Evans rats were examined in dissociated cell culture. Visual cortex from rat pups 1 to 15 days old was dissociated to yield a suspension of single cells. Neurons plated onto collagen or a feeder layer of astrocytes rapidly extended processes and survived for 4-10 weeks. Antisera to {dollar}gamma{dollar}-aminobutyric acid, glutamic acid decarboxylase, choline acetyltransferase and vasoactive intestinal polypeptide stained 24 {dollar}pm{dollar} 5%, 22 {dollar}pm{dollar} 2%, 2.3 {dollar}pm{dollar} 0.3% and 2.4 {dollar}pm{dollar} 0.2% of all neurons, respectively, suggesting that different neuronal classes survived roughly in proportion to their number in vivo.; Prior to dissociation, neurons in layer V of visual cortex that project to the superior colliculus were labeled in vivo by retrograde transport of fluorescent latex microspheres--a permanent fluorescent marker. Retrogradely labeled neurons were readily identified immediately after dissociation and throughout the period in vitro. After two weeks in culture, labeled cells exhibited many ultrastructural features characteristic of pyramidal neurons in vivo.; Intracellular recording techniques were used to evaluate the pharmacology of responses of cortical neurons to excitatory amino acids as well as the pharmacology of epsps. Glutamate, aspartate, N-methyl-D-aspartate (NMDA), kainate, and quisqualate excited every cortical neuron tested. Kynurenic acid, piperidine dicarboxylic acid and {dollar}gamma{dollar}-D-glutamylglycine anatagonized responses to all five agonists. These 3 drugs also completely blocked monosynaptic epsps produced by corticocollicular neurons and by most other excitatory neurons. APV, 2-amino-5-phonovaleric acid, selectively antagonized responses to NMDA and reduced polysynaptic excitation. In Mg{dollar}sp{lcub}2+{rcub}{dollar} free medium, most epsps exhibited a slow component that was blocked by APV. These results suggest that most excitatory cortical neurons in culture use an excitatory amino acid neurotransmitter. Epsps are largely mediated by non-NMDA receptors, but in Mg{dollar}sp{lcub}+2{rcub}{dollar} free medium most epsps exhibit a slow, APV-sensitive component attributable to the NMDA receptor.
机译：在分离的细胞培养中检查了来自产后Long Evans大鼠视觉皮层的神经元的特性。将来自1至15天大的幼崽的视觉皮层解离，以产生单细胞悬液。涂在胶原蛋白或星形胶质细胞饲养层上的神经元迅速扩展了过程，并存活了4-10周。对{美元}γ{美元}-氨基丁酸，谷氨酸脱羧酶，胆碱乙酰基转移酶和血管活性肠多肽的抗血清染色24 {美元} pm {美元} 5％，22 {美元} pm {美元} 2％，2.3 {美元} pm {dollar}分别占所有神经元的0.3％和2.4 {dollar} pm {dollar}占所有神经元的0.2％，这表明不同的神经元类别在体内存活的比例大致与其成比例。解离之前，通过逆向转运荧光乳胶微球体（一种永久性的荧光标记物）在体内标记出视觉皮层V层中突出到上丘的神经元。分离后立即以及在整个体外过程中都容易识别出逆行标记的神经元。培养两周后，标记的细胞在体内表现出许多锥体细胞特征的超微结构特征。细胞内记录技术用于评估皮质神经元对兴奋性氨基酸的反应的药理作用以及epsps的药理作用。谷氨酸，天门冬氨酸，N-甲基-D-天门冬氨酸（NMDA），海藻酸盐和喹喹啉兴奋每个被测皮层神经元。尿酸，哌啶二羧酸和{γ}γ{D} -D-谷氨酰甘氨酸对所有五个激动剂的反应都呈拮抗作用。这3种药物也完全阻断了皮质胶体神经元和大多数其他兴奋性神经元产生的单突触epsps。 APV 2-氨基-5-苯并戊酸选择性拮抗对NMDA的反应并减少多突触兴奋。在Mg {dollar} sp {lcub} 2+ {rcub} {dollar}游离培养基中，大多数epsps表现出缓慢的成分，被APV阻止。这些结果表明，培养物中大多数兴奋性皮质神经元都使用兴奋性氨基酸神经递质。 Epsps主要由非NMDA受体介导，但在不含Mg {dollar} sp {lcub} +2 {rcub} {dollar}的培养基中，大多数epsps均显示出归因于NMDA受体的APV敏感成分缓慢。

著录项

  • 作者

    HUETTNER, JAMES EDWARD.;

  • 作者单位

    Harvard University.;

  • 授予单位 Harvard University.;
  • 学科 Biology Neuroscience.
  • 学位 Ph.D.
  • 年度 1987
  • 页码 164 p.
  • 总页数 164
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 神经科学;
  • 关键词

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