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首页> 外文学位 >Novel syntheses of polyamines and the analysis of effects of substituted polyamines on protein and polyamine syntheses using Ps. (Kim) and Escherichia coli extracts.
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Novel syntheses of polyamines and the analysis of effects of substituted polyamines on protein and polyamine syntheses using Ps. (Kim) and Escherichia coli extracts.

机译：多胺的新型合成以及使用Ps分析取代的多胺对蛋白质和多胺合成的影响。 （金）和大肠杆菌提取物。

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摘要

The object of this study was twofold. One aspect of this work explored simple ways to synthesize substituted and unsubstituted polyamines. The other aspect of this work examined the effects of hydroxy and N-(amino) substituted polyamines on bacterial protein and polyamine syntheses.; Fairly facile syntheses of substituted and unsubstituted polyamines were accomplished using several methods. One novel approach, which lead to the generation of the widest array of polyamines, was a versatile reaction scheme involving a ring opening reaction of unsubstituted or N-substituted cyclic imides of different ring size with an amine or hydrazine to form the corresponding alkanediamide or hydrazidoalkanamide intermediates, which were ultimately reduced to polyamines. Another novel sequence used a synthetically specific step which led to the incorporation of a 2-hydroxybutanediamine segment in the ultimately formed polyamine. This sequence used 1,3-dipolar reactions to form specific structural intermediates which were ultimately reducible to the corresponding polyamines. One conventional sequence involved the conversion of substituted or unsubstituted diacids to diesters, which were converted to alkanediamides or dihydrazides; and then to diamines and dihydrazines. Another conventional sequence utilized amino cyanoethylation reactions, some of which could be used to form specific intermediates which were then reduced to polyamines.; A number of the synthesized hydroxyl and N-(amino) substituted polyamines were tested to determine their effect on bacterial protein and polyamine syntheses. The hydroxy but not N-(amino) substituted putrescine and spermidine analogues were generally capable of replacing the naturally occurring polyamines in the promotion of protein synthesis using E. coli or Ps. (Kim) extracts. The hydroxy and N-(amino) substituted polyamines were not substrates for S-adenosylmethionine decarboxylase (SAM DC) in the polyamine synthesis studies. Residual putrescine in the dialyzed polyamine synthesis extracts did react with the SAM DC in the presence of most of the polyamines except for N-(amino)putrescine and N,N{dollar}spprime{dollar}-bis(amino)putrescine. When equimolar amounts of C{dollar}sp{lcub}14{rcub}{dollar} labeled putrescine and one of derivatized polyamines were added to the polyamine synthesis system the labeled putrescine was converted to spermidine except in the presence of the N-(amino) substituted putrescines.
机译：这项研究的目的是双重的。这项工作的一个方面探索了合成取代和未取代的多胺的简单方法。这项工作的另一方面检查了羟基和N-（氨基）取代的多胺对细菌蛋白质和多胺合成的影响。使用几种方法可以轻松地完成取代和未取代的多胺的合成。导致生成最广泛的多胺阵列的一种新方法是一种通用的反应方案，该方案涉及将不同环尺寸的未取代或N-取代的环状酰亚胺与胺或肼进行开环反应，以形成相应的烷二酰胺或肼基烷酰胺中间体，最终被还原为多胺。另一个新颖的序列使用了合成特异性步骤，该步骤导致在最终形成的多胺中引入了2-羟基丁二胺链段。该序列使用1，3-偶极反应形成特定的结构中间体，该中间体最终可还原为相应的多胺。一种常规序列涉及将取代或未取代的二酸转化为二酯，再将其转化为链烷二酰胺或二酰肼。然后去二胺和二肼。另一种常规序列利用氨基氰乙基化反应，其中一些可用于形成特定的中间体，然后将其还原为多胺。测试了许多合成的羟基和N-（氨基）取代的多胺，以确定它们对细菌蛋白质和多胺合成的影响。羟基取代N-（氨基）取代的腐胺和亚精胺类似物通常能够代替天然存在的多胺，从而促进使用大肠杆菌或PS合成蛋白质。 （金）提取物。在多胺合成研究中，羟基和N-（氨基）取代的多胺不是S-腺苷甲硫氨酸脱羧酶（SAM DC）的底物。透析的多胺合成提取物中的残留腐胺在大多数多胺的存在下与SAM DC发生了反应，除了N-（氨基）putrescine和N，N {dol} spprime {dollar} -bis（bis）氨基。当等摩尔量的C {dollar} sp {lcub} 14 {rcub} {dollar}标记的腐胺和一种衍生化的多胺添加到多胺合成系统中时，除了在N-（氨基）存在下，标记的腐胺被转化为亚精胺。 ）取代的腐胺。

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  • 作者

    Parker, Raymond Samuel, III.;

  • 作者单位

    Rensselaer Polytechnic Institute.;

  • 授予单位 Rensselaer Polytechnic Institute.;
  • 学科 Chemistry Biochemistry.
  • 学位 Ph.D.
  • 年度 1987
  • 页码 300 p.
  • 总页数 300
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

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