...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Localization of polyamine enhancement of protein synthesis to subcellular components of Escherichia coli and Pseudomonas sp. strain Kim.
【24h】

Localization of polyamine enhancement of protein synthesis to subcellular components of Escherichia coli and Pseudomonas sp. strain Kim.

机译：定位多胺增强蛋白质合成至大肠杆菌和假单胞菌属sp的亚细胞成分。金应变。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

At 5 mM Mg2+, spermidine stimulation of polyphenylalanine synthesis by cell-free extracts of Escherichia coli was found to be about 30 times greater than that by extracts of Pseudomonas sp. strain Kim, a unique organism which lacks detectable levels of spermidine. By means of reconstitution experiments, the target of spermidine stimulation was localized to the protein fraction of the highspeed supernatant component (S-100) of E. coli and was absent from, or deficient in, the S-100 fraction of Pseudomonas sp. strain Kim. The spermidine stimulation did not appear to be due to the presence in the E. coli S-100 fraction of ribosomal protein S1, elongation factors, or E. coli aminoacyl-tRNA synthetases. The failure to observe spermidine stimulation by the Pseudomonas sp. strain Kim S-100 fraction was also not due to a spermidine-enhanced polyuridylic acid degradation. The synthesis of polyphenylalanine by Pseudomonas sp. strain Kim extracts was stimulated by putrescine and by S-(+)-2-hydroxyputrescine to a greater degree than was synthesis by E. coli extracts. The enhancement by putrescine and by S-(+)-2-hydroxyputrescine with Pseudomonas sp. strain Kim extracts was found to be due to effects on its ribosomes.
机译：发现在5 mM Mg2 +浓度下，亚精胺对大肠杆菌无细胞提取物的聚苯丙氨酸合成的刺激作用比假单胞菌sp。的提取物高约30倍。金菌株，一种独特的生物，缺乏可检测到的亚精胺水平。通过重构实验，亚精胺刺激的靶标定位于大肠杆菌的高速上清液组分（S-100）的蛋白质部分，并且在假单胞菌sp的S-100部分中缺乏或缺乏。金应变。亚精胺刺激似乎不是由于在大肠杆菌S-100级分中存在核糖体蛋白S1，延伸因子或大肠杆菌氨酰基-tRNA合成酶。未能观察到假单胞菌属物种对亚精胺的刺激。菌株Kim S-100部分也不是由于亚精胺增强的聚尿苷酸降解。假单胞菌（Pseudomonas sp。）合成聚苯丙氨酸。与大肠杆菌提取物合成相比，腐胺和S-（+）-2-羟基腐胺刺激金氏菌提取物的程度更大。腐胺和S-（+）-2-羟基腐胺与假单胞菌sp的增强作用。发现金氏提取物菌株是由于对其核糖体的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号