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首页> 外文学位 >Application of bovine rapid pregnancy test kit of the early pregnancy diagnosis in ewes in comparison with progesterone and pregnancy-specific protein B assays.
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Application of bovine rapid pregnancy test kit of the early pregnancy diagnosis in ewes in comparison with progesterone and pregnancy-specific protein B assays.

机译：与孕酮和妊娠特异性蛋白B分析相比，牛早期妊娠快速诊断测试试剂盒在母羊中的应用。

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摘要

The objective of the experiment was to identify the applicability of TARGET Bovine CL progesterone test kit (TARGET) for early pregnancy diagnosis in ewes. TARGET (BioMetallics, Inc.) is a commercial test kit to identify pregnancy in cows in the field. The test kit diagnostic result was assessed by standard color grade chart. When the blood or milk sample progesterone level is high, the test kit shows a white color and the cow is diagnosed as pregnant. The dark blue color indicates no-pregnancy, whereas the light blue and faint blue together indicate that there is no definite diagnosis, so a second test is required.In this experiment, 132 Merino ewes were divided into two groups. Eighty two ewes were assigned to a spring breeding flock at Rafter 7 Ranch (Yerington, NV), and the other 50 ewes were assigned to a fall breeding flock at Main Station Field Lab (MSFL, Reno, NV). In the Rafter 7 Ranch group, three diagnostic methods were applied to verify the reliability of the TARGET test kit: ELISA analysis of the blood progesterone level, ultrasound scanning for the pregnancy diagnosis, and the lambing records. An additional test called BioPRYN(Pregnancy Ruminant Yes/No) analysis, which tests PSPB(Pregnancy-Specific Protein B) level in the blood samples, was applied to the MSFL group.TARGET color grade readings, ELISA analysis, BioPRYN test results, ultrasound scanning results, and lambing records were analyzed by the FREQ procedure of the SAS (V 9.2) program to give the phi coefficients. For both groups, the cut-off value of ELISA progesterone assays to diagnose pregnancy in ewes was defined as >= 1 ng/ml. Six possible combinations within four color grades were evaluated separately. Sensitivity, specificity, and predictive values of TARGET kit color grade assignments for pregnancy diagnosis were calculated. For both groups, the color indicators of the TARGET test kit for early pregnancy diagnosis in ewes showed a significantly high phi coefficient (P0.0001), a correlative association measured based on the lambing records. In conclusion, based on the results above, the bovine TARGET test kit is practically applicable to the early pregnancy diagnosis in the field for ewes with a high degree of accuracy.
机译：实验的目的是确定TARGET牛CL孕酮检测试剂盒（TARGET）在母羊早期妊娠诊断中的适用性。 TARGET（BioMetallics，Inc.）是一种商业测试试剂盒，用于识别野外母牛的妊娠。通过标准颜色等级表评估测试套件的诊断结果。当血液或牛奶样品中的孕酮水平很高时，检测试剂盒将显示白色，并且该母牛被诊断为怀孕。深蓝色表示没有怀孕，而浅蓝色和淡蓝色一起表示没有确定的诊断，因此需要进行第二次测试。在此实验中，将132头美利奴羊母羊分为两组。在Rafter 7 Ranch（内华达州耶灵顿）的春季繁殖群中，有八十二头母羊被分配，在总部野外实验室（内华达州里诺市，MSFL），另外五十头母羊被归为秋季繁殖群。在Rafter 7 Ranch组中，采用了三种诊断方法来验证TARGET测试试剂盒的可靠性：血液孕酮水平的ELISA分析，用于怀孕诊断的超声扫描以及胎记。 MSFL组进行了另一项名为BioPRYN（妊娠反刍是/否）分析的测试，该测试用于检测血样中PSPB（妊娠特定蛋白B）的水平。目标颜色等级读数，ELISA分析，BioPRYN测试结果，超声扫描结果和羔羊记录通过SAS（V 9.2）程序的FREQ程序进行分析，以给出phi系数。对于两组，用于诊断母羊妊娠的ELISA孕酮检测的临界值均定义为> = 1 ng / ml。分别评估了四个颜色等级中的六个可能组合。计算了TARGET试剂盒颜色等级分配对妊娠诊断的敏感性，特异性和预测值。对于两组，用于母羊早期妊娠诊断的TARGET测试试剂盒的颜色指示器均显示出显着较高的phi系数（P <0.0001），这是根据成胎记录测得的相关性。总之，基于上述结果，牛TARGET检测试剂盒可高度准确地实际应用于母羊的早期妊娠诊断。

著录项

  • 作者

    Chen, Wei.;

  • 作者单位

    University of Nevada, Reno.;

  • 授予单位 University of Nevada, Reno.;
  • 学科 Biology Molecular.Agriculture Animal Culture and Nutrition.Agriculture General.
  • 学位 M.S.
  • 年度 2010
  • 页码 50 p.
  • 总页数 50
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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