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首页> 外文学位 >BACTERIOLOGIC, ECOLOGIC, SEROLOGIC, AND IMMUNOGENETIC STUDIES OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS - INDUCED CASEOUS LYMPHADENITIS IN SMALL RUMINANTS.
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BACTERIOLOGIC, ECOLOGIC, SEROLOGIC, AND IMMUNOGENETIC STUDIES OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS - INDUCED CASEOUS LYMPHADENITIS IN SMALL RUMINANTS.

机译：皮质反刍类细菌引起的小反刍类细菌性淋巴结杆菌的细菌学，生态学，血清学和免疫遗传学研究。

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摘要

Corynebacterium pseudotuberculosis-induced caseous lymphadenitis (CLA) is an economically important chronic disease of small ruminants characterized by abscessation of either or both lymph nodes or visceral organs. The concentration of C pseudotuberculosis in purulent exudate and the survival of the microorganism in this exudate was determined.;The survival of C pseudotuberculosis on or in a number of different fomites including inanimate surfaces (plastic, wood, and steel), particulate fomites (wood shavings, hay, straw, feces, and soil), and water was determined at 37, 22, and 4 C. The organism generally remained viable for longer time periods when CLA abscess contents were mixed with particulate fomites then when spread on surfaces. Incubation at lower temperatures generally, but not always, extended the survival period for the organism outside the host. Depending upon the source of water C pseudotuberculosis was able to remain viable for up to three days. The survival of C pseudotuberculosis in soil-purulent exudate mixtures was studied in both sterilized and unsterilized soil samples. The organism persisted in most sterilized soil samples for 6 months or more and in an unsterilized sandy loam soil for 3 to 7 weeks.;A bacterial agglutination test using a petroleum ether extracted sonicated cell wall antigen was developed for detecting antibodies against C pseudotuberculosis. In conjunction with bacteriologic and pathologic studies this serologic test was used to study prevalence of CLA in a population of mature culled sheep. Data from this study suggested that the bacterial agglutination serodiagnostic test may be useful as an in vitro immunodiagnostic test for ovine CLA. The relationship between ovine lymphocyte antigen (OLA) alleles and predisposition to C pseudotuberculosis-induced ovine CLA was investigated. These disease association studies revealed a relationship between certain OLA alleles and predisposition to either or both superficial and visceral forms of the disease. One OLA allele (214) was significantly associated with predisposition to all forms of CLA.;Subcutaneous challenge exposure of goats to 10('7) colony forming units of C pseudotuberculosis resulted in infection. The microflora of the experimentally induced chronic abscesses was evaluated 24 weeks later and the concentration of C pseudotuberculosis was determined. The concentration of the agent in axenic lesions ranged from 2.2 x 10('5) to 2.6 x 10('8) CFU/ml with an average of 2.9 x 10('7) CFU/ml, while in lesions with mixed microflora, it varied from 2.1 x 10('6) to 4.0 x 10('8) CFU/ml with an average of 7.0 x 10('7) CFU/ml.
机译：假结核杆菌诱发的干酪样淋巴结炎（CLA）是一种经济上重要的小反刍动物慢性疾病，其特征是淋巴结或内脏器官或两者均有脓肿。确定化脓性渗出液中C假结核的浓度以及该渗出液中微生物的存活率; C假结核在各种无毒物（无生物表面（塑料，木材和钢），无毒表面（木材）上或其中的存活率）分别在37、22和4 C下测定刨花，干草，稻草，粪便和土壤）和水。当CLA脓肿内容物与颗粒状的石棉混合后，再扩散到表面时，这种生物通常可以存活更长的时间。在较低温度下孵育通常（但不总是）延长了宿主外部生物的生存期。根据水的来源，假结核能够存活多达三天。在无菌和未灭菌的土壤样品中研究了化脓性渗出液混合物中假结核的存活。该微生物在大多数无菌土壤样品中持续存在6个月或更长时间，在未灭菌的沙质壤土中持续3至7周。结合细菌学和病理学研究，该血清学测试用于研究成年淘汰绵羊群体中CLA的患病率。这项研究的数据表明，细菌凝集血清诊断测试可以作为绵羊CLA的体外免疫诊断测试。研究了绵羊淋巴细胞抗原（OLA）等位基因与C假结核诱导的绵羊CLA易感性的关系。这些疾病关联研究揭示了某些OLA等位基因与该疾病的表皮和内脏形式的易感性之间的关系。一个OLA等位基因（214）与所有形式的CLA的易感性均显着相关。山羊皮下挑战暴露于10个（'7）C假结核菌落形成单位导致感染。 24周后评估实验诱导的慢性脓肿的菌群，并确定假结核的浓度。在病原体病变中，药剂的浓度范围为2.2 x 10（'5）至2.6 x 10（'8）CFU / ml，平均为2.9 x 10（'7）CFU / ml，而在混合微生物群的病变中，它从2.1 x 10（'6）到4.0 x 10（'8）CFU / ml不等，平均为7.0 x 10（'7）CFU / ml。

著录项

  • 作者

    AUGUSTINE, JOHN L.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Agriculture Animal Pathology.
  • 学位 Ph.D.
  • 年度 1984
  • 页码 156 p.
  • 总页数 156
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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