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首页> 外文学位 >NITRIFICATION--A POTENTIAL FOR HETEROTROPHIC NITRIFIERS FROM AN ACTIVATED SLUDGE SYSTEM (NITROGEN, SHOCK, LOADING).
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NITRIFICATION--A POTENTIAL FOR HETEROTROPHIC NITRIFIERS FROM AN ACTIVATED SLUDGE SYSTEM (NITROGEN, SHOCK, LOADING).

机译：硝化作用-活性污泥系统（氮，冲击，负荷）中异养亚硝化剂的潜力。

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Scope of Study. Studies involving the isolation of a heterotrophic nitrifier were undertaken. An enrichment technique was developed for the routine isolation procedures. This technique employed a medium with an organic compound as carbon and energy source and a supplementary substrate. Routine isolations were conducted for a period of 10 months during which time an activated sludge nitrification system was maintained for supplying the nitrifying seeds for isolation. A total of seventy cultures of heterotrophic organisms were isolated with the nitrifying enrichment media. Each isolate was individually tested for ability to form nitrite or nitrate in an acetate broth. Once the heterotrophic nitrifier was isolated, stock cultures of the isolate were maintained in shaken flasks for subsequent studies. Growth rate determinations were made for the culture under both carbon and nitrogen limiting conditions. A range of C:N ratios was selected for study on the substrate removal characteristics in batch systems. The system which promoted nitrification was later used to study the response to a glucose shock load.; Findings and Conclusions. A microorganism capable of utilizing organic carbon as carbon and energy source and capable of nitrifying has been isolated from an activated sludge system. An enrichment medium consisting of minimal salts, sodium acetate and yeast extract was found to enhance heterotrophic nitrification. Kinetic growth constants (mu)(,max) (0.37 hr('-1)) and K(,s) (220 mg/l) obtained under carbon limiting conditions for this microorganism are very typical of heterotrophic bacteria. This microorganism has been confirmed to nitrify heterotrophically by employing N-Serve, a potent inhibitor for the autotrophic nitrifier, Nitrosomonas. The insensitivity of the microorganism to this specific inhibitor and the inability to grow on sodium bicarbonate as sole source of carbon clearly suggests the physiological differences between heterotrophic and autotrophic nitrifiers. Under appropriate conditions of carbon and nitrogen supply especially when nitrogen is in excess of assimilatory needs, heterotrophic nitrification could be the predominant mode of mechanism in a biological nitrification system. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of author.) UMI
机译：研究范围。进行了涉及分离异养硝化器的研究。针对常规分离程序开发了一种浓缩技术。该技术采用了一种以有机化合物为碳源和能源的介质，以及一种辅助基质。常规分离进行了10个月，在此期间，维持了活性污泥硝化系统，以提供硝化种子进行分离。用硝化富集培养基分离出总共七十种异养生物。分别测试每个分离物在乙酸盐肉汤中形成亚硝酸盐或硝酸盐的能力。分离出异养硝化器后，将分离物的原种培养物置于摇瓶中，以备后续研究。在碳和氮限制条件下确定培养物的生长速率。选择了一系列C：N比率，以研究间歇系统中的基质去除特性。促进硝化作用的系统后来用于研究对葡萄糖休克负荷的反应。结论和结论。从活性污泥系统中分离出了能够利用有机碳作为碳和能源并能够硝化的微生物。发现由最少的盐，乙酸钠和酵母提取物组成的富集培养基可增强异养硝化作用。在该碳微生物的碳限制条件下获得的动力学生长常数（mu（，max）（0.37 hr（'-1））和K（，s）（220 mg / l）是异养细菌的典型特征。通过使用N-Serve（一种自养硝化硝化菌Nitrosomonas的有效抑制剂），已证实该微生物可进行异养硝化。微生物对这种特异性抑制剂的不敏感性以及不能以碳酸氢钠作为唯一碳源生长的现象清楚地表明了异养型和自养型硝化器之间的生理差异。在适当的碳和氮供应条件下，尤其是当氮超过同化需求时，异养硝化作用可能是生物硝化系统中主要的机制模式。 。 。 。 （作者的摘要超出了规定的最大长度。经作者许可，在此处停产。）UMI

著录项

  • 作者

    CHEAH, LAN SIEW.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Engineering Civil.
  • 学位 Ph.D.
  • 年度 1983
  • 页码 169 p.
  • 总页数 169
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 建筑科学;
  • 关键词

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