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首页> 外文学位 >Bi-parental mapping and genome-wide association studies for grain quality traits in winter wheat under contrasting soil moisture conditions.
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Bi-parental mapping and genome-wide association studies for grain quality traits in winter wheat under contrasting soil moisture conditions.

机译：在相反的土壤水分条件下，冬小麦籽粒品质性状的双亲作图和全基因组关联研究。

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摘要

Wheat grain quality is characterized by parameters such as grain protein concentration (Gpc), grain ash concentration (Gac), kernel weight (Kw), kernel diameter (Kd), and kernel hardness (Kh). Drought stress at flowering time can cause floral organ necrosis, thus, decreasing the number of grains per spike and filled grain percentage, while drought stress during grain filling reduces kernel weight and size, but increases grain protein concentration.;A previous study reported three chromosomal regions (1B, 6B, and 7B) associated with many quantitative trait loci (QTL) co-located for grain quality traits in a doubled haploid (DH) population derived from the cross CO940610/Platte. To validate those QTL, three objectives of this study were (1) QTL mapping in a CO940610/Platte recombinant inbred line (RIL) population, (2) transferring alleles of interest from CO940610 to the recurrent parent Platte by marker-assisted backcross (MABC), and (3) genome-wide association studies for grain yield (Gy), Gpc, grain protein deviation (Gpd), Gac, and test weight (Tw) in an association mapping panel.;A population of 186 CO940610/Platte RIL was grown in the Akron rainfed and Greeley fully irrigated environments in 2009/10. The same set of RIL was grown in a CSU Plant Sciences greenhouse for DNA extraction, and genotypes were obtained for 18 simple sequence repeat and sequence tagged site markers in three chromosome regions of interest. JoinMap 4.0 was used to construct linkage maps from the molecular marker data. Marker-trait associations (MTA) were detected by single-factor analysis of variance (ANOVA). Linkage maps constructed in the CO940610/Platte RIL and DH populations were mostly consistent. Most of the grain quality traits investigated were associated with the three chromosome regions on 1B, 6B, and 7B in at least one environment, confirming findings in the CO940610/Platte DH population.;Five selected DH lines and the recurrent parent Platte were used during MABC, resulting in 35 BC3F2 lines for field trials. These lines were classified into 8 allelic combinations at the selective marker loci Glu-B1, Xwmc182a, and Xwmc182b, representing of the regions of interest on chromosomes 1B, 6B, and 7B, respectively. Of these allelic combinations, lines having PL-PL-CO and CO-CO-PL at Glu-B1, Xwmc182a and Xwmc182b, respectively, were hypothesized to have the lowest and highest Gpc. Experiments for the 35 MABC lines were conducted in Fort Collins fully irrigated (sprinkler irrigation), Greeley irrigated (drip irrigation), and Greeley water deficit (severe stress during grain filling) environments. Marker-trait associations for Gpc detected at Xwmc182a and Xwmc182b in the BC 3F2 backcross population were consistent with findings in the CO940610/Platte DH population. The MTA for Gpc and Gac at locus Xwmc182a were robust across two of three environments. In the Fort Collins fully irrigated environment, Gpc of the allelic combination CO-CO-PL was significantly higher than the combination PL-PL-CO, confirming the hypothesized results.;A collection of 299 hard winter wheat cultivars and breeding lines representative of the U.S. Great Plains germplasm was evaluated for Gy, Gpc, grain protein deviation (Gpd), Gac and Tw. Experiments were designed as side-by-side moisture treatments in Greeley 2011/12 (drip irrigation, stress began pre-flowering) and Fort Collins 2012/13 (sprinkler irrigation, severe stress during grain filling). Each treatment was arranged as an augmented design with two check varieties, each check having 15 replicates. Grain protein concentration and Gpd were highly correlated (0.72 to 0.87, P<0.001) in all four environments. The panel was characterized using a high-density 90,000 gene-associated single nucleotide polymorphism (SNP) genotyping platform. After removing SNP that did not meet data quality criteria, 16,052 filtered SNP were used to perform the genome-wide association studies (GWAS) conducted in the R programming environment using the 'GAPIT' package. Principal components and a kinship matrix were incorporated to correct for population structure and relatedness among individuals. A total of 40 significant MTA (according to the significance threshold of P<1.67x10 -4, suggested by Gao et al. 2008) were detected for the five evaluated traits (Gy, Gpc, Gpd, Gac, and Tw). Of these, two SNP (BS00021704_51 and Excalibur_c4518_2931) on chromosome 6A were associated with Gy. The same SNP (BS00064369_51) on 4A was associated with both Gpc and Gpd. Test weight had the most MTA (17). Two SNP, BS00047114_51 and BS00065934_51, both associated with Tw on chromosome 3B, were robust across three of four environments investigated.;In conclusion, two narrow regions (~2 cM each) around Xwmc182a on 6B and Xwmc182b on 7B are of potential value for breeding programs. The incorporation of favorable allele combinations into a uniform background (Platte) was successful, but further investigation is needed for the MABC lines. Grain protein deviation appears to be a useful metric for increasing both Gpc and Gy. Five SNP should be investigated further to detect candidate genes in their respective chromosome regions. (Abstract shortened by UMI.).
机译：小麦籽粒品质的特征在于诸如谷物蛋白质浓度（Gpc），谷物灰分浓度（Gac），籽粒重量（Kw），籽粒直径（Kd）和籽粒硬度（Kh）之类的参数。开花期的干旱胁迫可导致花器官坏死，从而减少每个穗的籽粒数量和籽粒填充百分比，而籽粒填充期间的干旱胁迫则减少了籽粒的重量和大小，但增加了籽粒蛋白质的浓度。先前的研究报道了三种染色体与许多数量性状基因位点（QTL）相关联的区域（1B，6B和7B），这些基因位点来自从CO940610 / Platte杂交获得的双倍单倍体（DH）群体中的谷物品质性状。为了验证这些QTL，本研究的三个目标是（1）在CO940610 / Platte重组自交系（RIL）群体中进行QTL定位，（2）通过标记辅助回交（MABC）将感兴趣的等位基因从CO940610转移至复发的亲本Platte ），以及（3）在关联映射面板中对谷物产量（Gy），Gpc，谷物蛋白质偏差（Gpd），Gac和测试重量（Tw）进行全基因组关联研究；人口为186个CO940610 / Platte RIL在2009/10年度的Akron雨育和Greeley完全灌溉环境中种植。在CSU Plant Sciences温室中生长了相同的RIL集，用于DNA提取，并在三个感兴趣的染色体区域中获得了18个简单序列重复序列和序列标记位点标记的基因型。使用JoinMap 4.0从分子标记数据构建连锁图。通过单因素方差分析（ANOVA）检测标记-性状关联（MTA）。在CO940610 / Platte RIL和DH群体中构建的连锁图大部分是一致的。在至少一种环境中，大多数被调查的谷物品质性状都与1B，6B和7B上的三个染色体区域有关，从而证实了CO940610 / Platte DH群体的发现。 MABC，得到35条BC3F2品系用于田间试验。这些系在选择标记基因座Glu-B1，Xwmc182a和Xwmc182b处分为8个等位基因组合，分别代表染色体1B，6B和7B上的目标区域。在这些等位基因组合中，假设在Glu-B1，Xwmc182a和Xwmc182b分别具有PL-PL-CO和CO-CO-PL的品系具有最低和最高的Gpc。 35条MABC品系的实验是在柯林斯堡完全灌溉（喷灌），格里利灌溉（滴灌）和格里利缺水（谷物灌装过程中的严重压力）环境下进行的。 BC 3F2回交群体中在Xwmc182a和Xwmc182b处检测到的Gpc的标记-性状关联与CO940610 / Platte DH群体中的发现一致。 Xwmc182a基因座处的Gpc和Gac的MTA在三种环境中的两种环境中均很可靠。在柯林斯堡充分灌溉的环境中，等位基因组合CO-CO-PL的Gpc显着高于PL-PL-CO组合，证实了假设的结果。收集了299个硬冬小麦品种和育种系的代表对美国大平原种质进行了Gy，Gpc，谷物蛋白偏差（Gpd），Gac和Tw评估。实验被设计为在2011/12年度格里利（滴灌，应力开始开花前）和柯林斯堡2012/13中的并行水分处理（喷灌，谷物灌装过程中的严重压力）。每种处理均安排为带有两个检查品种的增强设计，每个检查具有15个重复项。在所有四种环境中，谷物蛋白浓度和Gpd高度相关（0.72至0.87，P <0.001）。该小组的特点是使用高密度90,000基因相关的单核苷酸多态性（SNP）基因分型平台。在删除了不符合数据质量标准的SNP之后，使用“ GAPIT”软件包在R编程环境中使用了16,052个经过过滤的SNP进行了全基因组关联研究（GWAS）。主要成分和亲属关系矩阵被合并以校正个体之间的人口结构和亲缘关系。对于五个评估的性状（Gy，Gpc，Gpd，Gac和Tw），共检测到40个显着的MTA（根据P <1.67x10 -4的显着性阈值，由Gao等人2008建议）。其中，染色体6A上的两个SNP（BS00021704_51和Excalibur_c4518_2931）与Gy相关。 Gpc和Gpd均与4A上的同一SNP（BS00064369_51）相关联。测试砝码的MTA最高（17）。两个SNP BS00047114_51和BS00065934_51都与3B染色体上的Tw相关联，在研究的四个环境中的三个环境中都很稳健;总之，在6B的Xwmc182a和7B的Xwmc182b周围有两个狭窄区域（每个〜2 cM）具有潜在的潜在价值繁殖计划。成功地将有利的等位基因组合整合到统一背景（Platte）中，但MABC线需要进一步调查。谷物蛋白质偏差似乎是增加Gpc和Gy的有用指标。应进一步研究五个SNP，以检测其各自染色体区域中的候选基因。 （摘要由UMI缩短。）。

著录项

  • 作者

    Dao, Hung Quoc.;

  • 作者单位

    Colorado State University.;

  • 授予单位 Colorado State University.;
  • 学科 Agriculture.;Biology.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 229 p.
  • 总页数 229
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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