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Fabrication of multibore carbon nanopipettes using a template-based nanomanufacturing process

机译：使用基于模板的纳米制造工艺制造多孔碳纳米移液管

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摘要

Currently, cancer accounts for nearly 1 of every 4 deaths in the United States which rates it as the second most common cause of death in the US, exceeded only by heart diseases. The detection, prevention and treatment of these death-causing diseases have necessitated and led to the development of novel tools for interfacing with single live cells --intracellular cell physiology. A major challenge, preventing the realization of effective and efficient intracellular physiology, is the lack of minimally invasive, nanoscale electrodes capable of probing cells without causing cell damage or death. Previous studies have succeeded in fabricating single nanoscale electrodes suitable for cell probing, but in this research, we introduce the controllable fabrication of Multibore Carbon Nanopipettes (MCNPs) --nanoscale probes with multiple, independent hollow carbon nanoscale electrodes within one very small tip -- in three stages: (i) forming templates by pulling micropipettes from theta glass capillaries (pipette pulling); (ii) selectively depositing carbon via CVD on the lumen walls of the micropipette (carbon deposition); and (iii) exposing the two carbon nanostructures formed at the micropipette tip with selective wet-etching (carbon exposure).;These MCNPs, suitable for cell probing, also incorporate a multifunctionality that is yet to be seen in existing microelectrodes. Here, we present the step-by-step, repeatable methodology in fabricating MCNPs, the governing parameters at different stages of fabrication and the effects of varying these parameters. We establish that the MCNP geometry can be defined at the pulling stage, where the taper length and diameter of the pipette have an inverse relationship; carbon thickness is defined at the carbon deposition stage and the carbon exposure stage defines the exposed carbon length. We also showed the capability of our MCNPs for intracellular injection by demonstrating their effectiveness in fluid transport and delivery. The fabrication technique offers a repeatable and low cost process of manufacturing MCNPs, thereby making it a commercially viable nanomanufacturing technique that will enable numerous intracellular applications beyond cell probing. Finally, the continuous development of the MCNPs for these numerous intracellular applications may bring about a reform in single cell analysis, biomedical research and disease pathology research.
机译：当前，癌症占美国每4例死亡中的近1例，这将其列为美国第二大最常见的死亡原因，仅次于心脏病。这些致死性疾病的检测，预防和治疗已成为必要，并导致开发了与单活细胞接口的新工具-细胞内细胞生理学。阻碍实现有效和有效的细胞内生理学的主要挑战是缺乏能够探测细胞而不引起细胞损伤或死亡的微创纳米级电极。先前的研究已经成功地制造出适用于细胞探测的单个纳米级电极，但是在这项研究中，我们介绍了可控制备多孔纳米碳纳米管（MCNPs）的方法-纳米探针在一个非常小的尖端内具有多个独立的空心碳纳米级电极-分三个阶段：（i）通过从theta玻璃毛细管中抽出微量移液器（移液器）形成模板； （ii）通过CVD在微量移液管的管腔壁上选择性地沉积碳（碳沉积）； （MC）这些适用于细胞探测的MCNP还具有多功能性，这在现有的微电极中尚不可见。在这里，我们介绍了制造MCNP的逐步，可重复的方法，在制造的不同阶段的控制参数以及改变这些参数的效果。我们确定可以在拉动阶段定义MCNP的几何形状，其中移液器的锥度长度和直径成反比关系。碳厚度在碳沉积阶段定义，碳暴露阶段定义暴露的碳长度。我们还通过证明MCNP在流体运输和输送中的有效性来展示其MCNP进行细胞内注射的能力。该制造技术提供了一种可重复且低成本的制造MCNP的方法，从而使其成为商业上可行的纳米制造技术，该技术将实现除细胞探测之外的许多细胞内应用。最后，针对这些众多细胞内应用的MCNP的不断发展可能会带来单细胞分析，生物医学研究和疾病病理学研究方面的变革。

著录项

  • 作者

    Arowosola, Ayomipo.;

  • 作者单位

    Rochester Institute of Technology.;

  • 授予单位 Rochester Institute of Technology.;
  • 学科 Biomedical engineering.;Mechanical engineering.;Nanotechnology.
  • 学位 M.S.
  • 年度 2015
  • 页码 101 p.
  • 总页数 101
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 公共建筑;
  • 关键词

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