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Successes and challenges in the development of baculovirus-based vaccines for swine influenza and porcine circovirus type 2b.

机译：在针对杆状病毒的2b型猪流感和猪圆环病毒疫苗开发中的成功与挑战。

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摘要

Respiratory disease is a major cause of economic losses for the swine industry and ranks as a primary contributor to mortality in swine. Over the last 20 years, as swine production operations have continued to consolidate, respiratory disease in swine has become increasingly multifactorial in nature. The work presented in this dissertation centers around the development of vaccines utilizing the baculovirus expression vector system (BEVS) for two viral pathogens associated with the Porcine Respiratory Disease Complex (PRDC), a multifactorial respiratory disease syndrome affecting growing and finishing phase pigs. The work in this dissertation demonstrates that vaccines formulated with subunit hemagglutinin (HA) fused to an IgG Fc domain, VLP-displayed HA, or baculovirus-displayed HA may be viable alternatives to the traditional inactivated whole virus approaches currently utilized in the veterinary vaccine industry. In addition, this work uncovers a potential problem for the development of VLP-based porcine circovirus type 2 (PCV2) vaccines as an ORF2 protein from an emerging PCV2b strain was found to have dramatically reduced yields of VLP when expressed using the BEVS. Furthermore, the work in this dissertation demonstrates that a single amino acid mutation can be used to overcome this loss of VLP yield and that the mutated ORF2 protein provides protective efficacy against a PCV2b challenge in pigs.
机译：呼吸系统疾病是养猪业经济损失的主要原因，是造成猪死亡率的主要因素。在过去的20年中，随着养猪生产活动的不断巩固，猪的呼吸道疾病在自然界中已变得越来越多。本论文的工作集中在利用杆状病毒表达载体系统（BEVS）开发与两种与猪呼吸道疾病综合症（PRDC）有关的病毒性病原体的疫苗有关，这是一种影响生长发育阶段猪的多因素呼吸道综合症。本文的工作表明，用融合到IgG Fc结构域的亚单位血凝素（HA），VLP展示的HA或杆状病毒展示的HA配制的疫苗可能是目前兽用疫苗行业中使用的传统灭活全病毒方法的可行替代品。 。此外，这项工作揭示了开发基于VLP的猪圆环病毒2型（PCV2）疫苗的潜在问题，因为当使用BEVS表达时，发现来自新兴PCV2b株的ORF2蛋白大大降低了VLP的产量。此外，本论文的工作表明，单个氨基酸突变可用于克服VLP产量的这种损失，并且突变的ORF2蛋白可提供抗猪​​PCV2b攻击的保护功效。

著录项

  • 作者

    Hernandez, Luis Alejandro.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Virology.;Veterinary science.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 109 p.
  • 总页数 109
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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