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High-throughput Microfluidic Assay Devices for Culturing of Soybean and Microalgae and Microfluidic Electrophoretic Ion Nutrient Sensor

机译：用于培养大豆和微藻的高通量微流分析仪以及微流电泳离子营养传感器

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摘要

In the past decade, there are significant challenges in agriculture because of the rapidly growing global population. Meanwhile, microfluidic devices or lab-on-a-chip devices, which are a set of micro-structure etch or molded into glass, silicon wafer, PDMS, or other materials, have been rapidly developed to achieve features, such as mix, separate, sort, sense, and control biochemical environment. The advantages of microfluidic technologies include high-throughput, low cost, precision control, and highly sensitive. In particular, they have offered promising potential for applications in medical diagnosis, drug discovery, and gene sequencing. However, the potential of microfluidic technologies for application in agriculture is far from being developed. This thesis focuses on the application of microfluidic technologies in agriculture. In this thesis, three different types of microfluidic systems were developed to present three approaches in agriculture investigation.;Firstly, this report a high throughput approach to build a steady-state discrete relative humidity gradient using a modified multi-well plate. The customized device was applied to generate a set of humidity conditions to study the plant-pathogen interaction for two types of soybean beans, Williams and Williams 82.;Next, a microfluidic microalgal bioreactor is presented to culture and screen microalgae strains growth under a set of CO2 concentration conditions. C. reinhardtii strains CC620 were cultured and screened in the customized bioreactor to validate the workability of the system. Growth rates of the cultured strain cells were analyzed under different CO2 concentrations. In addition, a multi-well-plate-based microalgal bioreactor array was also developed to do long-term culturing and screening. This work showed a promising microfluidic bioreactor for in-line screening based on microalgal culture under different CO2 concentrations.;Finally, this report presents a microchip sensor system for ions separation and detection basing electrophoresis. It is a system owning high potential in various ions concentration analysis with high specificity and sensitivity. In addition, a solution sampling system was developed to extract solution from the soil.;All those presented technologies not only have advantages including high-throughput, low cost, and highly sensitive but also have good extensibility and robustness. With a simple modification, those technologies can be expanded to different application areas due to experimental purposes. Thus, those presented microfluidic technologies provide new approaches and powerful tools in agriculture investigation. Furthermore, they have great potential to accelerate the development of agriculture.
机译：在过去的十年中，由于全球人口的快速增长，农业面临着巨大的挑战。同时，已经快速开发了微流体设备或芯片实验室设备，这些设备是一组微结构蚀刻或模制成玻璃，硅片，PDMS或其他材料，可以实现混合，分离等功能。 ，分类，感知和控制生化环境。微流体技术的优势包括高通量，低成本，精确控制和高度灵敏。特别是，它们为医学诊断，药物发现和基因测序提供了广阔的应用前景。但是，微流体技术在农业中的应用潜力还远远没有得到开发。本文主要研究微流控技术在农业中的应用。本文研究了三种不同类型的微流体系统，提出了三种农业研究方法。首先，本研究提出了一种高通量方法，采用改进的多孔板构建稳态离散相对湿度梯度。使用定制的设备生成一组湿度条件，以研究两种类型的大豆（Williams和Williams 82）的植物与病原体的相互作用。二氧化碳浓度条件。在定制的生物反应器中培养和筛选莱茵衣藻CC620菌株，以验证系统的可操作性。在不同CO 2浓度下分析培养的菌株细胞的生长速率。此外，还开发了基于多孔板的微藻生物反应器阵列，以进行长期培养和筛选。这项工作显示了一种有前途的微流控生物反应器，可用于在不同CO2浓度下基于微藻培养物进行在线筛选。最后，本报告提出了一种用于电泳分离和检测离子的微芯片传感器系统。该系统在各种离子浓度分析中具有很高的潜力，具有很高的特异性和灵敏度。此外，还开发了一种从土壤中提取溶液的溶液采样系统。所有这些提出的技术不仅具有高通量，低成本和高度灵敏的优点，而且具有良好的可扩展性和鲁棒性。通过简单的修改，由于实验目的，这些技术可以扩展到不同的应用领域。因此，那些提出的微流体技术为农业研究提供了新的方法和强大的工具。此外，它们具有加速农业发展的巨大潜力。

著录项

  • 作者

    Xu, Zhen.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Engineering.
  • 学位 Ph.D.
  • 年度 2018
  • 页码 124 p.
  • 总页数 124
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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