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首页> 外文学位 >Biodetection and Biointerface Based on Nanostructured Aluminum Oxide (NAO): From Fluorescence Enhancement to MS Effect on Single Neural Cells
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Biodetection and Biointerface Based on Nanostructured Aluminum Oxide (NAO): From Fluorescence Enhancement to MS Effect on Single Neural Cells

机译：基于纳米氧化铝（NAO）的生物检测和生物接口：从荧光增强到单细胞神经质谱的影响

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摘要

Anodic aluminum oxide (AAO) has been investigated and utilized in numerous products for almost a century. But the rapidly increasing interest in nanoscale materials and their outstanding properties has propelled nanoporous AAO to be used as a substrate for sensors and biosensors. Fluorescence-based biosensors are one type of optical biosensors which are very popular for detecting a variety of targets (DNA, RNA, glucose, enzyme, bacteria, etc.). The recent discovery of AAO fluorescence enhancement effect makes AAO more attractive since it has the great potential to be used in biosensing field to improve the sensor sensitivity. However, the mechanism of the AAO fluorescence enhancement effect has not be understood thoroughly. Based on the experimental and modeling results, it has been found that the main contributing factor to the fluorescence enhancement is probably the plasmonic Al nanoparticles (NPs) embedded in the film, while the nanopore dimensions have a limited contribution. Based on its fluorescence enhancement effect, a new class of molecular beacon biosensors is developed to detect specific hairpin DNA sequence. The sensor demonstrates excellent specificity and selectivity, indicating the great promise of this type of sensor for diagnostic applications. Furthermore, another optical biosensor has been developed based on AAO. TGF-beta1 which is one type of growth factor secreted by pancreatic stellate cell (ITAF) has successfully detected by this sensor. It has been found that 10 ng/ml of purified transforming growth factor beta1 (TGF-beta1) can be readily detected in buffer with high specificity. TGF-beta1 in a conditioned cell medium has also been detected successfully. By comparing with the reference data of purified TGF-beta1, concentration of TGF-beta1 secreted in the conditioned cell medium has been reasonable estimated. Finally, Transcranial magnetic stimulation (MS) effects on single neuron cell (N27) have been studied on both glass and AAO substrate. It has been found that MS not only has a negligible cytotoxic effect on N27 cells but also can speed up the N27 cell proliferation and regeneration.
机译：阳极氧化铝（AAO）在近一个世纪的时间里已被研究并用于多种产品中。但是，人们对纳米级材料及其出色性能的兴趣迅速增长，促使纳米多孔AAO用作传感器和生物传感器的基材。基于荧光的生物传感器是一种光学生物传感器，非常流行于检测各种目标物（DNA，RNA，葡萄糖，酶，细菌等）。 AAO荧光增强作用的最新发现使AAO更具吸引力，因为它在生物传感领域具有巨大的潜力，可以提高传感器的灵敏度。然而，尚未完全了解AAO荧光增强作用的机理。根据实验和建模结果，发现导致荧光增强的主要因素可能是包埋在薄膜中的等离子Al纳米颗粒（NPs），而纳米孔尺寸的贡献有限。基于其荧光增强作用，开发了一种新型的分子信标生物传感器来检测特定的发夹DNA序列。该传感器具有出色的特异性和选择性，表明这种类型的传感器可用于诊断应用。此外，已经开发了基于AAO的另一种光学生物传感器。 TGF-beta1是由胰腺星状细胞（ITAF）分泌的一种生长因子，已通过该传感器成功检测到。已经发现，可以在具有高特异性的缓冲液中容易地检测到10ng / ml的纯化的转化生长因子β1（TGF-β1）。还已成功检测到条件细胞培养基中的TGF-beta1。通过与纯化的TGF-β1的参考数据进行比较，可以合理地估计条件细胞培养基中分泌的TGF-β1的浓度。最后，在玻璃和AAO基质上都研究了经颅磁刺激（MS）对单个神经元细胞（N27）的作用。已经发现MS不仅对N27细胞具有可忽略的细胞毒性作用，而且可以加速N27细胞的增殖和再生。

著录项

  • 作者

    Che, Xiangchen.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Electrical engineering.;Bioengineering.;Optics.
  • 学位 Ph.D.
  • 年度 2018
  • 页码 133 p.
  • 总页数 133
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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