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Microfluidic Study of Hydrogen Peroxide (H2O 2) Transport Modeled on the MSN-DA Neuron Pathway

机译：以MSN-DA神经元途径为模型的过氧化氢（H2O 2）转运的微流研究

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摘要

Reactive Oxygen Species (ROS) are produced throughout the body and can cause damage, lead to neurodegenerative disorders, and deactivate neurons involved in the release of essential neurotransmitters. However, the underlying mechanisms affecting neuronal dysfunction are controversial and are not yet well understood. Hydrogen peroxide (H2O2), a common ROS, has been shown to inhibit evoked dopamine (DA) in the Nigrostriatal region of the brain. Although unconfirmed, one theory claims that the DA-modulating H2O2 is produced in medium spiny neurons (MSN), based on close proximity. However, most literature cites H2O2 as a very small molecule which can travel far, diffuse readily, and transport easily between cells. This theory is commonly used when describing ROS damage, but more insight is needed to be able to distinguish mass transport pathways of hydrogen peroxide. The experimental setup used in this research was developed around a sensitive, cost-effective, reliable, solution for detecting H 2O2. A microfluidic device was designed to simulate the basic geometry of the MSN-DA pathway and was fabricated using 3-D printing. Sample collection and colorimetric analysis was fine-tuned so that a time-dependent analysis of H2O2 transport was possible, within the limitations of the system. This work represents a proof-of-concept scenario and information gained can be used for future experiments aimed at predicting H2O2 transport within the MSN-DA pathway.
机译：人体产生活性氧（ROS），会造成损害，导致神经退行性疾病，并使参与释放必需神经递质的神经元失活。但是，影响神经元功能障碍的潜在机制尚有争议，尚未得到很好的理解。过氧化氢（H2O2）是一种常见的ROS，已被证明可以抑制大脑黑质纹状体区域中诱发的多巴胺（DA）。尽管尚未得到证实，但一种理论声称，基于紧密邻近性，DA调节型H2O2是在中棘神经元（MSN）中产生的。但是，大多数文献都将H2O2称为非常小的分子，它可以传播很远，很容易扩散并且可以在细胞之间轻松运输。在描述ROS损伤时通常使用该理论，但是需要更多的见识来区分过氧化氢的质量传递途径。本研究中使用的实验装置是围绕一种灵敏，经济高效，可靠的H 2 O 2检测解决方案开发的。设计了一种微流体装置来模拟MSN-DA路径的基本几何形状，并使用3-D打印技术制造。对样品的收集和比色分析进行了微调，以便可以在系统限制内进行随时间变化的H2O2传输分析。这项工作代表了概念验证的场景，所获得的信息可用于未来的实验，旨在预测MSN-DA途径内的H2O2传输。

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  • 作者

    Phillips, Carrie A.;

  • 作者单位

    Rochester Institute of Technology.;

  • 授予单位 Rochester Institute of Technology.;
  • 学科 Mechanical engineering.;Neurosciences.
  • 学位 M.S.
  • 年度 2018
  • 页码 58 p.
  • 总页数 58
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 公共建筑;
  • 关键词

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