• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Optimization of Human Adipose-derived Stem Cell (ADSC) Culture for Growth, Multi-lineage Differentiation and Regenerative Potential.
【24h】

Optimization of Human Adipose-derived Stem Cell (ADSC) Culture for Growth, Multi-lineage Differentiation and Regenerative Potential.

机译：人类脂肪干细胞（ADSC）培养物的生长，多谱系分化和再生潜能的优化。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Adipose tissue is rich with stem cells and harvesting these cells is minimally invasive to donors, making it an ideal tissue to work with. Stem cells have the capacity to maintain prolonged self-renewal, as well as the ability to differentiate into cells with more specialized functions. Given their unique regenerative abilities, adipose-derived mesenchymal stem cells (ADSCs) offer new potentials for use in clinical therapy and regenerative medicine. Optimizing ADSC growth for therapeutic use involves both rapid expansion of cells and cellular characterization including the growth factor/ cytokine secretome that may be crucial to promote healing.;The first part of this study was to optimize stem cell growth using xeno-free medium. We grew cells in DMEM/F12 with 1% human serum (HS), 2.5% HS 10% fetal bovine serum (FBS) with and without FGF-2, with or without ITS, on uncoated polysterile or pre-coated fibronectin plates. FGF-2 was essential for growth in low-serum conditions while pre-coated fibronectin plates provided minimal increases. Doubling times on uncoated plates were 26.7 hours for 1%HS+FGF, 25.1 hours for 1%HS+FGF+ITS, and 26.7 hours for 2.5%HS+FGF and 17.9 hours for 10%FBS+FGF.;Additionally we evaluated the regenerative potential of ADSCs culture-expanded under different conditions by comparing the ratio of gene expression of hepatic growth factor (HGF) to the expression of transforming growth factor (TGF-beta) by quantitation with RT-qPCR. The HGF/TGFB1 ratio was 20.5 for 1%HS+FGF, 23.8 for 1%HS+FGF+ITS, 2.0 for 10%FBS+FGF and 0.1 for 10%FBS noFGF.;Further evaluation of gene expression, of ADSCs culture-expanded in 1%HS+FGF, 1%HS+FGF +ITS, 10%FBS+FGF, and 10%FBS noFGF, was done using an Affymetrix microarray. Significant differences in pathway-dependent gene expression between 10% FBS and 1%HS conditions were reflected in the ability of the stem cells to undergo differentiation into osteogenic, chondrogenic, adipogenic and myogenic lineages. 1%HS+FGF produced the best osteogenic differentiation whereas 10%FBS noFGF produced the best chondrogenic differentiation. The variation in gene expression observed between the growth conditions may help to predict the most efficient conditions to build tissue models for future genetic disease research and/or clinical drug screening and development.
机译：脂肪组织富含干细胞，收获这些细胞对供体的侵害微乎其微，使其成为理想的组织。干细胞具有维持长时间自我更新的能力，并具有分化成具有更专门功能的细胞的能力。鉴于其独特的再生能力，源自脂肪的间充质干细胞（ADSC）为临床治疗和再生医学提供了新的潜力。优化用于治疗用途的ADSC生长涉及细胞的快速扩增和细胞特性的鉴定，包括可能对促进愈合至关重要的生长因子/细胞因子分泌基因组。这项研究的第一部分是使用无异种培养基优化干细胞的生长。我们在未包被的无菌或预包被的纤连蛋白平板上，在含有1％人血清（HS），2.5％HS 10％胎牛血清（FBS）的DMEM / F12中，在有或没有FGF-2的情况下，在有或没有ITS的情况下培养细胞。 FGF-2对于在低血清条件下的生长至关重要，而预涂的纤连蛋白平板提供的增幅很小。对于1％HS + FGF，未涂层平板的加倍时间是26.7小时，对于1％HS + FGF + ITS是25.1小时，对于2.5％HS + FGF是26.7小时，对于10％FBS + FGF是17.9小时。通过RT-qPCR定量比较肝生长因子（HGF）基因表达与转化生长因子（TGF-beta）表达的比率，可在不同条件下培养ADSCs的再生潜力。对于1％HS + FGF，HGF / TGFB1的比率为20.5，对于1％HS + FGF + ITS，HGF / TGFB1的比率为23.8，对于10％FBS + FGF，HGF / TGFB1的比率为2.0，对于10％FBS noFGF，HGF / TGFB1的比率为0.1。使用Affymetrix微阵列进行1％HS + FGF，1％HS + FGF + ITS，10％FBS + FGF和10％FBS noFGF扩增的扩增。干细胞经历分化为成骨，成软骨，成脂和成肌谱系的能力反映了10％FBS和1％HS条件之间途径依赖性基因表达的显着差异。 1％HS + FGF产生最佳的成骨分化，而10％FBS noFGF产生最佳的成软骨分化。在生长条件之间观察到的基因表达的变化可能有助于预测最有效的条件，以建立组织模型以用于未来的遗传疾病研究和/或临床药物筛选和开发。

著录项

  • 作者

    Perkail, Stephanie.;

  • 作者单位

    University of Maryland, Baltimore.;

  • 授予单位 University of Maryland, Baltimore.;
  • 学科 Health Sciences Epidemiology.;Biology Cell.;Biology Genetics.;Health Sciences Immunology.
  • 学位 M.S.
  • 年度 2014
  • 页码 109 p.
  • 总页数 109
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 地球物理学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号