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首页> 外文学位 >Quartz crystal microbalance (QCM) measurements for rapidly predicting toxin induced cell death.
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Quartz crystal microbalance (QCM) measurements for rapidly predicting toxin induced cell death.

机译：石英晶体微量天平（QCM）测量用于快速预测毒素诱导的细胞死亡。

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摘要

Piezoelectric quartz systems have historically been used in analytical chemistry because their oscillating frequency value is sensitive and directly proportional to the crystal mass. Adding mass to the crystal decreases the frequency and reducing mass increases it. This mass sensing ability has resulted in the creation of a sensing device known as the quartz crystal microbalance (QCM). Since the QCM detects any mass coupled to its surface, it has been widely used as a label-free signal transduction platform for chemical sensor and biosensor designs. Recently, cell-based biosensors have been developed. In these devices, living cells are attached to the gold surface of the QCM and serve as the sensing element, where cellular mass and viscoelastic properties affect the oscillation frequency of the crystal. The advantage of cells as the sensing element is that they possess a wide range of intelligent system properties resulting from the interplay of their integral membrane receptor-cytoskeletal-nuclear membrane systems to alter their mass distribution or viscoelastic properties due to external (i.e. toxins) or internal (i.e. mitochondria activity) signals. Thus, cells can detect a wide variety of changes in their environment, from individual molecules to more complex alterations. These intelligent properties can be exploited when cells are used as biosensor detection elements.;In a series of studies developing cell-based biosensors, we can detect effects of small molecules that affect specific aspects of the cells. Advantages of live cell biosensors are that they measure continuously in real time. The unique attributes of QCM cell-based sensors allow them to detect agents in an environment, such as engineered nanomaterials (ENMs). Because the QCM measures a direct effect of the nanomaterial on living cells this avoids complications of ENMs interference with reporters in assays. The output is gathered continuously so unique cellular response information, such as brief transient exposures are detected and the kinetics of cell stress and recovery can be detected.;The use of a mass measuring device incorporating living cells as the sensing component can therefore provide important new response information and overcome inherent confounding issues of conventional toxicity testing methods.
机译：压电石英系统在历史上一直用于分析化学，因为它们的振荡频率值很敏感，并且与晶体质量成正比。向晶体增加质量会降低频率，降低质量会增加频率。这种质量感测能力已导致创建了一种称为石英微天平（QCM）的感测设备。由于QCM可以检测耦合到其表面的任何质量，因此已被广泛用作化学传感器和生物传感器设计的无标记信号转导平台。最近，已经开发了基于细胞的生物传感器。在这些设备中，活细胞附着在QCM的金表面并用作传感元件，其中细胞质量和粘弹性质会影响晶体的振荡频率。细胞作为传感元件的优势在于，它们具有广泛的智能系统特性，这是由于它们的整体膜受体-细胞骨架-核膜系统相互影响而改变的，这是由于外部（即毒素）或内部（线粒体活动）信号。因此，细胞可以检测到环境中的各种变化，从单个分子到更复杂的变化。当将细胞用作生物传感器检测元件时，可以利用这些智能特性。在开发基于细胞的生物传感器的一系列研究中，我们可以检测影响细胞特定方面的小分子的作用。活细胞生物传感器的优点是它们可以实时连续测量。基于QCM细胞的传感器的独特属性使它们能够检测环境中的物质，例如工程纳米材料（ENM）。由于QCM可以测量纳米材料对活细胞的直接作用，因此可以避免ENM干扰测定过程中报道分子的复杂性。不断收集输出信息，因此可以检测到独特的细胞反应信息，例如短暂的短暂暴露，并可以检测细胞应激和恢复的动力学。因此，使用结合活细胞作为传感组件的质量测量设备可以提供重要的新发现。响应信息，并克服了常规毒性测试方法固有的混淆性问题。

著录项

  • 作者

    Dewilde, Abiche Helen.;

  • 作者单位

    University of Massachusetts Lowell.;

  • 授予单位 University of Massachusetts Lowell.;
  • 学科 Engineering Biomedical.;Biology General.
  • 学位 Ph.D.
  • 年度 2014
  • 页码 309 p.
  • 总页数 309
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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