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Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.

机译:猪黄体生成素对奶牛卵泡内环境和颗粒细胞和卵母细胞基因表达的影响。

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摘要

In previous research, the use of porcine luteinizing hormone (pLH) in lieu of gonadotropin-releasing hormone (GnRH) for synchronizing ovulation in a fixedtimed artificial insemination protocol in dairy cows improved pregnancy rates (42 vs 28%) without increasing progesterone concentrations. The mechanisms associated with this increase in pregnancy rates are not known. We hypothesized that exposure to pLH alters intrafollicular protein/gene expression compared to GnRH treatment, particularly the oocyte-expressed members of the TGF-beta superfamily, and EGF-like growth factor, which regulate oocyte competence. To address this, two studies were conducted to determine whether replacing pLH with GnRH to synchronize ovulation in dairy cows altered intrafollicular milieu and gene expression in mural granulosa cells and oocyte cumulus complexes. The results indicated that the relative abundance of BMP-15, GDF-9 and TGF-beta1 were greatly increased in pLH-treated cows. Since these factors promote cumulus expansion and oocyte competence, they may have contributed to the pregnancy increase previously reported.
机译:在先前的研究中,使用猪促黄体生成激素(pLH)代替促性腺激素释放激素(GnRH)在固定时间的人工授精方案中同步排卵可提高奶牛的妊娠率(42%对28%),而不会增加孕激素浓度。与妊娠率增加相关的机制尚不清楚。我们假设,与GnRH处理相比,暴露于pLH会改变卵泡内蛋白/基因表达,特别是卵母细胞表达的TGF-β超家族成员和EGF样生长因子,它们调节卵母细胞的能力。为了解决这个问题,进行了两项研究,以确定是否用GnRH替代pLH以同步奶牛的排卵,从而改变了壁内颗粒细胞和卵母细胞卵丘复合物的卵泡内环境和基因表达。结果表明,在经pLH处理的奶牛中,BMP-15,GDF-9和TGF-beta1的相对丰度大大提高。由于这些因素促进了卵丘扩张和卵母细胞能力,因此它们可能导致了先前报道的妊娠增加。

著录项

  • 作者

    Behrouzi, Amir.;

  • 作者单位

    University of Alberta (Canada).;

  • 授予单位 University of Alberta (Canada).;
  • 学科 Animal sciences.
  • 学位 M.S.
  • 年度 2014
  • 页码 139 p.
  • 总页数 139
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 老年病学;
  • 关键词

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