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Design and Fabrication of Nanodevice for Cell Interfacing.

机译：用于细胞接口的纳米器件的设计和制造。

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摘要

The goal of this thesis is to (a) design and fabricate a nanodevice that interface with cells and (b) optimize neuronal cell culturing protocol. The long term objective of this thesis is to perform intracellular electrical signal recording and stimulation of neuronal cells. To achieve this objective, a nanodevice with "Fin" shaped electrodes was designed that increases the electrode area and conductance so that it reduces the signal loss as shown in the case of traditional circular Nanopillar design. The overarching goal of neuroscience is to target and discover the relationships between the functional connectivity-map of neuronal circuits and their physiological or pathological functions. For recording large number of neurons, technologies such as gold mushroom-shaped microelectrodes (Hai et al.), vertical nanowire electrode arrays (VNEAs) (Robinson et al.) and nanoFET technology (Tian et al.) are currently under development. The gold mushroom-shaped electrodes in order of microns are invasive for smaller cells with no successful recording for long durations. The VNEAs show high electrode impedance which causes large signal loss. The nanoFET shows higher noise levels and the manipulation of a single nanotube to penetrate a single cell is very challenging. This thesis presents the design and fabrication of a "Fin" shaped nanoelectrode which seeks to overcome the restrictions between electrode impedance and electrode size. Compared to the 3x3 array of 200nm diameter nanowire electrodes, the "Fin" electrodes reduces the interfacial impedance. The fabrication was done in Silicon on insulator wafer with conducting lines and contact pads completely insulated by Silicon dioxide layer and gold coated nanofins. Nanofins of width 200 nm were fabricated using Focused Ion Beam (FIB) milling. Both high density and low density nanofins were optimized and cells were cultured over them. The optimization of cell culture, adherence and differentiation protocols were done to grow cells over the nanofins.
机译：本论文的目的是（a）设计和制造与细胞接口的纳米器件，（b）优化神经元细胞培养方案。本论文的长期目标是进行细胞内电信号记录和神经细胞刺激。为了实现该目标，设计了具有“ Fin”形电极的纳米器件，该器件增加了电极面积和电导率，从而减少了信号损耗，如传统的圆形纳米柱设计所示。神经科学的总体目标是针对并发现神经元回路的功能连接图与其生理或病理功能之间的关系。为了记录大量的神经元，目前正在开发诸如金蘑菇形微电极（Hai等），垂直纳米线电极阵列（VNEAs）（Robinson等）和nanoFET技术（Tian等）的技术。微米级的金蘑菇形电极对较小的细胞具有侵入性，长期无法成功记录。 VNEA显示出高电极阻抗，这会导致较大的信号损耗。 nanoFET显示出更高的噪声水平，并且操纵单个纳米管穿透单个电池非常具有挑战性。本论文提出了“ Fin”形纳米电极的设计和制造，其旨在克服电极阻抗和电极尺寸之间的限制。与200nm直径的纳米线电极的3x3阵列相比，“ Fin”电极减小了界面阻抗。该制造是在绝缘体晶片上的硅上完成的，其导线和接触垫完全被二氧化硅层和镀金纳米鳍片绝缘。使用聚焦离子束（FIB）研磨制造了宽度为200 nm的纳米鳍。优化了高密度和低密度纳米鳍，并在其上培养了细胞。对细胞培养，粘附和分化方案进行了优化，以使细胞在纳米鳍上生长。

著录项

  • 作者

    Chinnappan, Prema.;

  • 作者单位

    North Carolina Agricultural and Technical State University.;

  • 授予单位 North Carolina Agricultural and Technical State University.;
  • 学科 Nanotechnology.;Nanoscience.
  • 学位 M.S.
  • 年度 2014
  • 页码 80 p.
  • 总页数 80
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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