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首页> 外文学位 >Generation and Characterization of Tomato Lines with Modified Acylsugar and Fatty Acid Profiles
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Generation and Characterization of Tomato Lines with Modified Acylsugar and Fatty Acid Profiles

机译：修饰的糖和脂肪酸谱的番茄品系的产生和鉴定

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摘要

Acylsugars are accumulated by several wild relatives of tomato and are implicated in mediating a variety of defensive plant-insect interactions. Acylsugars are produced at high levels by glandular trichomes of Solanum pennellii and the ability to produce acylsucrose acylsugars at elevated levels was previously bred into tomato using S. pennellii (Correll) D'Arcy accession LA716, leading to the creation of the benchmark line CU071026, which exhibits an acylsugar chemotype profile distinct from that of S. pennellii LA716.;Four QTL previously associated with changes in fatty acid profile (FA2, FA5, FA7, and FA8), were individually combined with the five introgressions of CU071026. Characterization of the resulting lines indicated alterations to acylsugar level and acylsugar chemotype by modulating the length, orientation and/or relative proportion of fatty acid acyl groups, leading to changes in overall acylsugar composition.;The combinatory effect of three of the QTL (FA2, FA7, and FA8) on acylsugar level and chemotype was evaluated by combining these QTL. Characterization of the resulting lines demonstrated that the fatty acid QTL interacted additively and epistatically to alter acylsugar level and chemistry, increasing the diversity of acylsugars accumulated. The acylsugar level and chemotype traits of the lines generally displayed high heritability and minimal environmental interaction.;Three acylglucose QTL (AG3, AG4, AG11), previously associated with control of acylsugar moiety, were utilized to generate lines with acylglucose accumulation. Two approaches were taken to generate these lines and characterization demonstrated that epistatic interactions among the three acylglucose QTL controlled acylsugar level and acylglucose accumulation and that the three QTL can result in moderate levels of acylglucoses, but that an additional QTL might be needed for high acylglucose accumulation. Additionally, characterization of the acylsugar chemistry revealed impact of the acylglucose QTL on the composition of accumulated acylsucroses, and potential impact on the fatty acid composition.;The knowledge and the germplasm from this dissertation will support the generation of high acylsugar-accumulating tomato lines and hybrids with increased acylsugar profile diversity. This germplasm can also contribute to elucidation of the mechanism of insect resistance mediated by acylsugars, and assist with identification of yet-unknown acylsugar synthesis genes.
机译：酰基糖由番茄的几个野生近缘种积累而成，并参与介导各种防御性植物-昆虫相互作用。茄子的毛状腺毛高产糖基糖，以前使用S. pennellii（Correll）D'Arcy加入LA716将高水平生产酰基蔗糖糖基糖的能力培育到番茄中，从而产生了基准线CU071026，表现出与糖蔗糖LA.716不同的酰基糖化学型；先前与脂肪酸谱变化相关的四个QTL（FA2，FA5，FA7和FA8）分别与CU071026的五个渗入结合在一起。所得谱线的表征表明，通过调节脂肪酸酰基的长度，方向和/或相对比例，酰基糖水平和酰基糖化学型发生了变化，从而导致了总体酰基糖成分的变化。三个QTL（FA2，通过结合这些QTL来评估酰基糖水平和化学型的FA7和FA8）。所得品系的表征表明，脂肪酸QTL加性和上位性相互作用以改变酰基糖的水平和化学性质，从而增加了积累的酰基糖的多样性。品系的酰基糖水平和化学型性状通常表现出高遗传力和最小的环境相互作用。以前与酰基糖部分的控制有关的三个酰基葡萄糖QTL（AG3，AG4，AG11）被用于产生具有酰基葡萄糖积累的品系。采取了两种方法来生成这些谱线，并且表征证明三个酰基葡萄糖QTL之间的上位相互作用控制了糖的糖水平和酰基葡萄糖的积累，并且三个QTL可以导致中等水平的酰基葡萄糖的水平，但是可能需要额外的QTL来实现较高的酰基葡萄糖的积累。此外，对酰基糖化学特性的表征揭示了酰基葡萄糖QTL对积累的酰基蔗糖组成的影响以及对脂肪酸组成的潜在影响。；本文的知识和种质将支持高酰基糖积累的番茄品系和酰基糖谱多样性增加的杂种。该种质还可以有助于阐明由酰基糖介导的昆虫抗性的机制，并有助于鉴定尚未发现的酰基糖合成基因。

著录项

  • 作者

    Smeda, John Reid.;

  • 作者单位

    Cornell University.;

  • 授予单位 Cornell University.;
  • 学科 Plant sciences.;Biochemistry.;Agriculture.;Genetics.
  • 学位 Ph.D.
  • 年度 2017
  • 页码 194 p.
  • 总页数 194
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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