• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Characterization and analysis of secondary metabolites from foliar fungal endophytes of spruce trees.
【24h】

Characterization and analysis of secondary metabolites from foliar fungal endophytes of spruce trees.

机译：云杉树木叶片真菌内生菌次生代谢产物的表征和分析。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A collection of more than 2000 endophytes were isolated from natural white (Picea glauca) and red (Picea rubens) spruce trees located in New Brunswick, Nova Scotia and Maine, USA. A random selection of both white and red spruce endophytes were grown in one litre fermentations. Strains of interest due to their ability to grow and produce substantial amounts of secondary metabolites under laboratory conditions, were further screened by LC-MS. The extracts that showed interesting peaks in the chromatograms were tested for toxicity to spruce budworm ( Choristoneura fumiferana) using diet incorporated bioassays and for antifungal properties to Saccharomyces cerevisae using disc diffusion testing. The taxonomy of these strains that displayed toxicity were then examined using DNA sequencing to try and determine the identity of the endophytes present in the needles.;This study had two primary foci; the first was the characterization and structure elucidation of secondary metabolites from foliar fungal endophytes from both white and red spruce trees using MS and NMR. The work was highlighted by the discovery of two novel secondary metabolites, compounds 1 and 2a; two new fungal metabolites, compounds 3 and 4; and the known metabolites 5-10 from the three strains of white spruce endophytes studied in detail. Additionally, compounds 11 and 15 are reported as new structures, 12 and 13 as natural products for the first time, 14 is reported here for the first time as a fungal metabolite and 16-22 were identified as known fungal metabolites in new genera, from the six strains of red spruce endophytes examined. The second focus was on the analysis of the toxins and the presence of the endophyte in inoculated seedlings. Antibody based ELISA methods were developed for the detection of five white spruce endophytes in needles, to provide a reliable method for determining inoculation success. Analytical methods were also developed using HPLC and LC-MS/MS in multiple reaction monitoring (MRM) mode to determine the presence and concentration of the characterized toxins in infected tree needles for three of the white spruce endophytes. The results indicated that needles of trees colonized with these endophytes contain the toxins. These toxins in turn are responsible for reducing the growth rate of spruce budworm larvae. The presence of the endophyte and its toxin is thought to increase the development time of larvae, put the populations out of reproductive synchrony and expose them to a greater risk of death from parasitoides, pathogens and predation.
机译：从位于美国新斯科舍省和美国缅因州新不伦瑞克的天然白云杉（Picea glauca）和红云杉（Picea rubens）分离出2000多种内生菌。白色和红色云杉内生菌的随机选择均在一升发酵中生长。由于其在实验室条件下能够生长和产生大量次生代谢产物的能力，它们可以通过LC-MS进一步筛选。使用掺入饮食的生物测定法测试了在色谱图中显示出有趣峰的提取物对云杉芽虫（Choristoneura fumiferana）的毒性，并使用圆盘扩散测试对酿酒酵母（Saccharomyces cerevisae）的抗真菌特性进行了测试。然后使用DNA测序技术对这些表现出毒性的菌株进行分类，以试图确定针中存在的内生菌的身份。首先是使用MS和NMR对白云杉和红云杉树的叶真菌内生菌次级代谢产物进行表征和结构解析。通过发现两种新的次生代谢产物化合物1和2a突显了这项工作。两种新的真菌代谢物化合物3和4;并详细研究了三种云杉内生菌菌株的已知代谢物5-10。此外，化合物11和15被首次报道为新结构，12和13首次被报道为天然产物，14在这里首次被报道为真菌代谢产物，16-22被鉴定为新属的已知真菌代谢产物。检查了六种红色云杉内生菌。第二个重点是分析接种苗中的毒素和内生菌的存在。开发了基于抗体的ELISA方法，用于检测针中的五种白云杉内生菌，以提供确定接种成功的可靠方法。还使用HPLC和LC-MS / MS在多反应监测（MRM）模式下开发了分析方法，以确定三种白云杉内生菌在感染的树针中特征毒素的存在和浓度。结果表明，被这些内生菌定植的针叶中含有毒素。这些毒素反过来会降低云杉芽虫幼虫的生长速度。认为内生菌及其毒素的存在会增加幼虫的发育时间，使种群失去生殖同步性，并使他们面临更多因寄生虫，病原体和被捕食而死亡的风险。

著录项

  • 作者

    Sumarah, Mark William.;

  • 作者单位

    Carleton University (Canada).;

  • 授予单位 Carleton University (Canada).;
  • 学科 Chemistry Analytical.;Chemistry Organic.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 213 p.
  • 总页数 213
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;有机化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号