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Molecular and genetic analysis of symbiosis expressed secondary metabolite genes from the mutualistic fungal endophytes Neotyphodium lolii and Epichloe festuca

机译:共生的分子和遗传分析表达了来自互动真菌内生细胞新磷酸萝莉和孢子生成的二次代谢物基因

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Epichloe endophytes (Epichloe j Neotyphodium species) are an important group of clavicipitaceous fungi that form symbiotic associations (symbiota) with temperate grasses of the Pooideae subfamily (Scott, 2001a; Schaidl, Leuchtmann & Spiering, 2004). These biotrophic fungi systemically colonize the intercellular spaces of leaf primordia, leaf sheaths and culms of vegetative tissue and the inflorescence of reproductive tissues. The asexual Neotyphodium species have no external growth stage and consequently form symptomless associations with their grass host where they are vertically transmitted through the seed following colonization of the developing ovule. Most of the sexual Epichloe species are also transmitted vertically but because of their ability to form external stromata on inflorescence tissue, can also be transmitted horizontally (Leuchtmann & Schardl, 1998; Schardl & Leuchtmann, 1999). The mating system is hetero-thallic (outcrossing) and in nature is mediated by anthomyiid flies {Botanophila spp.), which transfer spermatia between stromata (Bultman et al., 1995; Bultman et al, 1998). Formation of perithecia and release of ascospores into the environment provides a source of vegetative hyphae that give rise to conidia that subsequently infect new hosts, probably by way of colonization of the stigma and style of the host inflorescence (Chung & Schardl, 1997).
机译:Epichloe Endophytes(Epichloe J新苯二苯硫胺类物种)是一组重要的克拉维哌菌真菌,形成共生协会(Symbiota),其温带Poo eae Subfamily(Scott,2001a; Schaidl,Leuchtmann&Spiering,2004)。这些生物营养真菌系统地定植了植物组织的叶子原基,叶子护套和秆的细胞间隙和生殖组织的花序。无性的新羟基物质没有外部生长阶段,因此与他们的草宿主形成症状关联,其中它们在显影胚瓣的定殖之后通过种子垂直传播。大多数性癫痫种也垂直透过,但由于它们在花序组织上形成外部stromata的能力,也可以水平传播(Leuchtmann&Schardl,1998; Schardl&Leuchtmann,1999)。交配系统是异质 - 铊(outcrossing),并且本质上由苍蝇(Botanophila SPP)介导的,其在stromata(Bultman等,1995)之间转移精子; Bultman等,1998)。终结性的形成和释放囊孢子进入环境的源泉提供了一种植物菌根来源,其产生随后感染新宿主的分类,可能是通过定位耻辱和宿主开花的风格(Chung&Schardl,1997)。

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