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首页> 外文学位 >Variation in biomass composition and regulation of lignocellulosic deposition in shrub willow (Salix ssp.) bioenergy crops.
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Variation in biomass composition and regulation of lignocellulosic deposition in shrub willow (Salix ssp.) bioenergy crops.

机译：灌木柳（Salix ssp。）生物能源作物中生物量组成的变化和木质纤维素沉积的调节。

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摘要

Genetic improvement of fast-growing shrub willow (Salix spp.), a proven perennial bioenergy crop for temperate climates, will require methods for rapid determination of biomass composition and will be expedited by a thorough understanding of the molecular basis for cell wall composition. High resolution thermogravimetric analysis (HR-TGA) is a reliable technique to identify differences in stem biomass composition among shrub willow varieties produced through breeding and has been developed for rapid, low-cost trait phenotyping and selection of shrub willow genotypes with optimized biomass composition. To validate the HR-TGA method, a selection of 25 shrub willow varieties was analyzed using traditional wet chemistry techniques and the results were compared by regression analysis to the results of HR-TGA. Correlations of the percent hemicellulose, cellulose, and lignin were all significant with R2 values over 0.7. Furthermore, the HR-TGA method was able to identify compositional differences among various age classes of willow and biomass samples with and without bark. The molecular basis for differences in biomass composition is being investigated by studying the expression of genes encoding enzymes involved in lignin biosynthesis and selected carbohydrate active enzymes (CAZymes). Fragments of nearly 20 genes have been cloned from willow cDNA and their expression patterns associated with stem growth throughout the growing season were analyzed by probe-based, real-time qPCR. Correlating patterns of gene expression with variation in biomass composition among selected shrub willow varieties and siblings within a hybrid family will provide insights into the genetic regulation of lignocellulosic deposition in this important bioenergy crop.;Key Words: bioenergy, biofuels, cell wall biosynthesis, cellulose, hemicelluloses, lignin, thermogravimetric analysis
机译：快速生长的灌木柳树（Salix spp。）的遗传改良是一种成熟的多年生生物能源作物，适合温带气候，将需要快速测定生物量组成的方法，并且通过对细胞壁组成的分子基础的透彻了解将得到加速。高分辨率热重分析（HR-TGA）是一种可靠的技术，可识别通过育种生产的灌木柳树品种之间茎生物量组成的差异，并且已开发用于快速，低成本性状表型鉴定和选择具有最佳生物量组成的灌木柳树基因型。为了验证HR-TGA方法，使用传统的湿化学技术分析了25种灌木柳品种，并通过回归分析将结果与HR-TGA进行了比较。半纤维素，纤维素和木质素百分比的相关性均显着，R2值超过0.7。此外，HR-TGA方法能够识别出有和没有树皮的不同年龄类别的柳树和生物量样品之间的成分差异。通过研究编码参与木质素生物合成的酶和选择的碳水化合物活性酶（CAZymes）的基因的表达，研究了生物量组成差异的分子基础。从柳树cDNA中克隆了近20个基因的片段，并通过基于探针的实时qPCR分析了它们在整个生长季节与茎生长相关的表达模式。在杂种科中，选定的灌木柳品种和兄弟姐妹之间基因表达模式与生物量组成的变化相关，将为这一重要生物能源作物中木质纤维素沉积的遗传调控提供见解。关键词：生物能源，生物燃料，细胞壁生物合成，纤维素，半纤维素，木质素，热重分析

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  • 作者

    Serapiglia, Michelle Joy.;

  • 作者单位

    State University of New York College of Environmental Science and Forestry.;

  • 授予单位 State University of New York College of Environmental Science and Forestry.;
  • 学科 Biology Botany.;Biology Plant Physiology.;Biology Genetics.;Alternative Energy.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 290 p.
  • 总页数 290
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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