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Characterizing zebrafish maternal effect genes involved in cytokinesis and axis formation.

机译：表征斑马鱼母体效应基因参与胞质分裂和轴形成。

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摘要

The zebrafish has emerged as a powerful system to study a variety of biological processes. Studying maternal effect mutants in zebrafish provides a great tool to dissect the molecular mechanisms of maternal control in vertebrate development. Here we present the characterization of positional cloning of two zebrafish maternal effect mutations, one in the gene nebel that affects cytokinesis, and another one in the gene hecate that affects dorsal axis formation.;nebel is a recessive, strictly maternal gene involved in cytokinesis. nebel mutant embryos show a dramatic reduction in the intensity of the slow calcium waves that accompany furrow formation. nebel mutant embryos show cytokinesis defects in cleavage stages as well as later defects in dorsal gene expression and convergence extension. Positional cloning indicates that the mutation in nebel is associated with the substitution of a highly conserved amino acid in the gene L-aspartate dehydrogenase. Studies of this gene in bacteria indicate that it may be involved in the synthesis of key players in calcium signaling.;hecate mutation results in ventralized embryos that have defects in the formation of dorsoanterior structures. hecate mutant embryos have reduced expression of genes specific to the dorsal organizer. Positional cloning reveals that hecate encodes glutamate receptor interacting protein 2 (Grip2) and is essential for dorsal determination. Zebrafish grip2 mRNA localizes to one side of the vegetal pole of the yolk during the zygote and cleavage stages before midblastula transition. In hecate mutant embryos, grip2 mRNA expression is strongly reduced. Further studies of this gene will provide great insight to our understanding of zebrafish early dorsal determination.
机译：斑马鱼已经成为研究各种生物过程的强大系统。对斑马鱼母体效应突变体的研究为剖析脊椎动物发育中母体控制的分子机制提供了一个很好的工具。在这里，我们介绍了两个斑马鱼母体效应突变的位置克隆的特征，其中一个是影响细胞分裂的nebel基因突变，另一个是影响背轴形成的基因hecate突变; nebel是一个隐性的，严格的母体基因，参与细胞分裂。 nebel突变胚胎在伴随沟形成的慢钙波强度上显着降低。 nebel突变体胚胎在分裂阶段表现出胞质分裂缺陷，而在背侧基因表达和会聚扩展方面表现出后期缺陷。位置克隆表明nebel中的突变与L-天冬氨酸脱氢酶基因中高度保守的氨基酸的取代有关。在细菌中对该基因的研究表明，它可能参与了钙信号传导的关键参与者的合成； hecate突变导致腹侧胚胎的背侧前侧结构形成存在缺陷。 hecate突变体胚胎的背组织器特异基因的表达减少。位置克隆显示，乙酸盐编码谷氨酸受体相互作用蛋白2（Grip2），对于确定背位至关重要。斑马鱼的grip2 mRNA在中胚层过渡之前的合子和卵裂期定位在蛋黄的植物极的一侧。在hecate突变体胚胎中，hip2 mRNA表达大大降低。对该基因的进一步研究将为我们对斑马鱼早期背侧测定的理解提供很大的见识。

著录项

  • 作者

    Ge, Xiaoyan.;

  • 作者单位

    The University of Wisconsin - Madison.;

  • 授予单位 The University of Wisconsin - Madison.;
  • 学科 Biology Genetics.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 92 p.
  • 总页数 92
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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