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首页> 外文学位 >Exploration of the RNase III dependent nuclear processing and degradation mechanisms of RNA in Saccharomyces cerevisiae.
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Exploration of the RNase III dependent nuclear processing and degradation mechanisms of RNA in Saccharomyces cerevisiae.

机译：探索酿酒酵母中RNA酶III依赖性RNA的核加工和降解机理。

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摘要

RNA molecules have had a very important role in living organisms, both past and present. Maintaining the integrity of RNA molecules through RNA surveillance is an essential process which involves a number of RNA endoribonucleases as well as RNA exoribonucleases. RNase III enzymes perform double-stranded RNA cleavage through which they assist in the processing and degradation of many types of RNA molecules. The S. cerevisiae ortholog of RNase III, Rnt1p, takes part in many of these processing events by cleaving noncoding RNAs such as rRNA, snRNAs and snoRNAs. Additionally, Rnt1p also takes part in RNA surveillance by facilitating the degradation of a number of noncoding RNAs as well as mRNAs and pre-mRNAs. Here we show that a number of cleavage intermediates of Rnt1p become 3' polyadenylated by RNA surveillance mechanisms involving the Trf4p poly(A) polymerase, and are subsequently further processed or degraded by the nuclear exosome. In addition, we show that the mating-type specific gene MATa1, expressed in MATa and in diploid cells, is regulated by nuclear RNA surveillance mechanisms. RNA forms resulting from variant splicing of the pre-MATa1 mRNA containing two introns are targeted for degradation through the 5' to 3' exonuclease Xrn1p and Rat1p, the RNase III endonuclease, and possibly the Nonsense Mediated Decay pathway. These novel examples of regulation through RNA processing and RNA decay underscore the importance of post-transcriptional processes in eukaryotic gene regulation.
机译：RNA分子在过去和现在的生物中都具有非常重要的作用。通过RNA监测维持RNA分子的完整性是一个必不可少的过程，其中涉及许多RNA内切核糖核酸以及RNA外切核糖核酸酶。 RNase III酶可进行双链RNA切割，从而协助多种类型的RNA分子的加工和降解。 RNase III的酿酒酵母直系同源基因Rnt1p通过切割非编码RNA（如rRNA，snRNA和snoRNA）参与了许多此类加工过程。此外，Rnt1p还通过促进许多非编码RNA以及mRNA和pre-mRNA的降解来参与RNA监测。在这里，我们显示Rnt1p的许多裂解中间体通过涉及Trf4p poly（A）聚合酶的RNA监测机制变成3'聚腺苷酸化，随后被核外泌体进一步加工或降解。此外，我们表明，在MATa和二倍体细胞中表达的交配型特异性基因MATa1受核RNA监视机制的调节。包含两个内含子的pre-MATa1 mRNA的可变剪接产生的RNA形式将通过5'至3'核酸外切酶Xrn1p和Rat1p，RNase III核酸内切酶以及可能的无义介导的衰变途径降解。这些通过RNA加工和RNA降解进行调控的新颖例子强调了转录后过程在真核基因调控中的重要性。

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  • 作者

    Egecioglu, Defne Emel.;

  • 作者单位

    University of California, Los Angeles.;

  • 授予单位 University of California, Los Angeles.;
  • 学科 Biology Molecular.;Chemistry Biochemistry.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 183 p.
  • 总页数 183
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:38:19

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