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首页> 外文期刊>Journal of Molecular Biology >E. coli RpsO mRNA decay: RNase E processing at the beginning of the coding sequence stimulates poly(A)-dependent degradation of the mRNA.
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E. coli RpsO mRNA decay: RNase E processing at the beginning of the coding sequence stimulates poly(A)-dependent degradation of the mRNA.

机译：大肠杆菌RpsO mRNA降解：编码序列开始时的RNase E加工刺激了poly（A）依赖的mRNA降解。

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摘要

The rpsO mRNA of E. coli encoding ribosomal protein S15 is destabilized by poly(A) tails posttranscriptionally added by poly(A)polymerase I. We demonstrate here that polyadenylation also contributes to the rapid degradation of mRNA fragments generated by RNase E. It was already known that an RNase E cleavage occurring at the M2 site, ten nucleotides downstream of the coding sequence of rpsO, removes the 3' hairpin which protects the primary transcript from the attack of polynucleotide phosphorylase and RNase II. A second RNase E processing site, referred to as M3, is now identified at the beginning of the coding sequence of rpsO which contributes together with exonucleases to the degradation of messengers processed at M2. Cleavages at M2 and M3 give rise to mRNA fragments which are very rapidly degraded in wild-type cells. Poly(A)polymerase I contributes differently to the instability of these fragments. The M3-M2 internal fragment, generated by cleavages at M3 and M2, is much more sensitive to poly(A)-dependent degradation than the P1-M2 mRNA, which exhibits the same 3' end as M3-M2 but harbours the 5' end of the primary transcript. We conclude that 5' extremities modulate the poly(A)-dependent degradation of mRNA fragments and that the 5' cleavage by RNase E at M3 activates the chemical degradation of the rpsO mRNA. Copyright 1999 Academic Press.
机译：编码核糖体蛋白S15的大肠杆菌的rpsO mRNA被poly（A）聚合酶I转录后添加的poly（A）尾巴破坏了稳定性。已知在rpsO编码序列下游10个核苷酸的M2位点发生的RNase E裂解除去了3'发夹，该发夹保护了初级转录本免受多核苷酸磷酸化酶和RNase II的攻击。现在，在rpsO编码序列的开始处确定了另一个称为M3的RNase E处理位点，该位点与核酸外切酶一起促进了在M2处理的信使的降解。在M2和M3处的切割产生了在野生型细胞中非常迅速降解的mRNA片段。聚（A）聚合酶I对这些片段的不稳定性有不同的贡献。通过在M3和M2处裂解产生的M3-M2内部片段比P1-M2 mRNA对poly（A）依赖的降解更敏感，P1-M2 mRNA具有与M3-M2相同的3'末端，但具有5'主要成绩单的末尾。我们得出的结论是5'末端调节了mRNA片段的poly（A）依赖性降解，并且RNase E在M3处的5'裂解激活了rpsO mRNA的化学降解。版权所有1999，学术出版社。

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