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首页> 外文学位 >Comparative transcriptomics in vector-rickettsiae interactions: New insights into molecular interactions between Rickettsia typhi and its insect vector.
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Comparative transcriptomics in vector-rickettsiae interactions: New insights into molecular interactions between Rickettsia typhi and its insect vector.

机译：载体-立克次体相互作用中的比较转录组学：伤寒立克次体与其昆虫载体之间分子相互作用的新见解。

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摘要

Rickettsia typhi is an obligate intracellular bacterium, and the causative agent of murine typhus, a flea-borne febrile illness prevalent and endemic in coastal areas throughout the world. Unlike most vector-borne pathogens, rickettsiae have the unique ability to persist in their vector host. Thus, once the infection is established, infected arthropods may serve as reservoirs of rickettsiae in nature. The goal of this study was to examine global transcription profiles of both R. typhi and its flea vector, Ctenocephalides felis, to elucidate molecular patterns underlying the tight associations between the two organisms. We hypothesize that in order to establish a persistent infection, specific changes in gene expression of both R. typhi and its flea vector, C. felis, are elicited in response to each other. This intricate molecular balancing act likely involves R. typhi-directed events to allow for successful infection of the flea midgut, as well as concomitant flea responses to limit the infection. Microarray analysis was used to uncover differences in global transcription profiles of R. typhi in response to a down-shift in temperature in vitro, mimicking one of the environmental changes encountered by R. typhi as it transitions from the mammalian host to the flea vector. This study identified differential transcription of genes from various functional groups, including genes that encode a putative rickettsial type IV secretion system, as well as upregulation of a rickettsial gene encoding a hypothetical protein (RT0279). Transcriptional analysis indicates that RT0279 is co-transcribed with downstream rickettsial vir-homologs, and when expressed in E. coli, is secreted into the extracellular milieu. Additionally, these studies compared the global transcription profiles of R. typhi infected and uninfected C. felis midguts to identify candidate flea midgut genes involved in the flea response to R. typhi infection. Follow up studies identified significant differences in gene expression of putative C. felis proteases and GTPases over an infection time course in vivo.
机译：伤寒立克次体是专性的细胞内细菌，是鼠伤寒的病原体，鼠伤寒是一种跳蚤传播的发热性疾病，在全世界沿海地区普遍流行。与大多数媒介传播的病原体不同，立克次体具有在其媒介宿主中持久存在的独特能力。因此，一旦感染被建立，被感染的节肢动物就可以充当自然界立克次体的宿主。这项研究的目的是检查伤寒沙门氏菌及其跳蚤载体Ctenocephalides felis的总体转录谱，以阐明这两种生物之间紧密联系的分子模式。我们假设，为了建立持续的感染，伤寒沙门氏菌及其跳蚤载体C. felis的基因表达都发生了特定变化，以响应彼此。这种错综复杂的分子平衡作用可能涉及伤寒沙门氏菌定向事件，以使跳蚤中肠成功感染，并伴随跳蚤反应以限制感染。微阵列分析被用于揭示鼠伤寒沙门氏菌响应于体外温度的下移而在全局转录谱中的差异，模仿了鼠伤寒沙门氏菌从哺乳动物宿主过渡到跳蚤载体时所遇到的环境变化之一。这项研究确定了来自各个功能组的基因的差异转录，包括编码假定的IV型立克次体分泌系统的基因，以及编码假想蛋白（RT0279）的立克次体基因的上调。转录分析表明，RT0279与下游立克次氏体病毒同系物共转录，当在大肠杆菌中表达时，被分泌到细胞外环境中。此外，这些研究比较了感染伤寒沙门氏菌和未感染伤寒沙门氏菌中肠的全局转录谱，以鉴定参与对伤寒沙门氏菌感染的跳蚤反应的候选跳蚤中肠基因。后续研究发现，在体内的感染过程中，假定的假丝酵母蛋白酶和GTPases基因表达存在显着差异。

著录项

  • 作者

    Dreher-Lesnick, Sheila M.;

  • 作者单位

    University of Maryland, Baltimore.;

  • 授予单位 University of Maryland, Baltimore.;
  • 学科 Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 202 p.
  • 总页数 202
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

  • 入库时间 2022-08-17 11:38:28

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