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首页> 外文学位 >Analysis of the amino-terminal domain of Drosophila Rbf1 indicates novel roles in cell regulation.
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Analysis of the amino-terminal domain of Drosophila Rbf1 indicates novel roles in cell regulation.

机译：果蝇Rbf1的氨基末端域的分析表明在细胞调节中的新作用。

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摘要

The retinoblastoma tumor suppressor protein (RB) is an important regulator of the cell cycle and development. Significantly, RB is inactivated in a majority of human cancers. Thus, elucidating the function of RB will give us a better understanding of how it prevents cancer. Many decades of research have yielded a detailed understanding of the role of RB in cell proliferation through transcriptional repression of target genes. However, the precise mechanisms of its action in many cellular pathways are poorly understood, including the control of DNA replication and post-transcriptional control of gene expression. Drosophila melanogaster presents a simplified genetic system to study cancer genes. Several published observations have suggested a role for RB in regulating DNA replication. Interestingly, other data indicate that RB associates with RNA processing factors. I have characterized novel protein-protein interactions with the Drosophila retinoblastoma tumor suppressor homologue Rbf, with an emphasis on its poorly characterized N-terminal domain. I describe the interaction of Rbf with the origin recognition complex, indicating a unique connection to DNA replication control. I also show that Rbf interacts with the RNA binding protein Squid, and review the literature that suggests potential role of RB/E2F in the control of RNA processing. The ability to control RNA processing may be an additional, unappreciated mode of gene regulation by RB. A focused study of the uncharacterized amino-terminal domain of Rbf has revealed new details about the retinoblastoma tumor suppressor in cell regulation, including DNA replication and RNA processing.
机译：视网膜母细胞瘤肿瘤抑制蛋白（RB）是细胞周期和发育的重要调节剂。值得注意的是，RB在大多数人类癌症中均失活。因此，阐明RB的功能将使我们对它如何预防癌症有更好的了解。数十年的研究已经通过对靶基因的转录抑制，深入了解了RB在细胞增殖中的作用。但是，人们对其作用在许多细胞途径中的确切机制知之甚少，包括对DNA复制的控制和对基因表达的转录后控制。果蝇（Drosophila melanogaster）提供了简化的遗传系统来研究癌症基因。几篇已发表的观察结果提示RB在调节DNA复制中的作用。有趣的是，其他数据表明RB与RNA加工因子有关。我已经表征了与果蝇视网膜母细胞瘤抑癌同源物Rbf的新型蛋白质-蛋白质相互作用，重点是其表征较差的N末端结构域。我描述了Rbf与起源识别复合物的相互作用，表明了与DNA复制控制的独特联系。我还表明Rbf与RNA结合蛋白Squid相互作用，并回顾了表明RB / E2F在控制RNA加工中的潜在作用的文献。控制RNA加工的能力可能是RB对基因进行调控的另一种未认识的模式。对Rbf的未表征的氨基末端结构域的集中研究揭示了视网膜母细胞瘤肿瘤抑制因子在细胞调控方面的新细节，包括DNA复制和RNA加工。

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  • 作者

    Ahlander, Joseph Andrew.;

  • 作者单位

    The University of Arizona.;

  • 授予单位 The University of Arizona.;
  • 学科 Biology Molecular.;Biology Genetics.;Biology Cell.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 91 p.
  • 总页数 91
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;细胞生物学;遗传学;
  • 关键词

  • 入库时间 2022-08-17 11:38:25

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