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Systematics of Neotropical Psiguria (Cucurbitaceae): Identifying low-copy nuclear markers, molecular phylogenetics, and taxonomic revision.

机译：新热带银屑病（葫芦科）的系统学：鉴定低拷贝核标记，分子系统发育学和分类学修订。

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摘要

Psiguria Arn. is a small genus of Neotropical vines in the Cucurbitaceae that grows in both wet and dry tropical forests from southern Mexico to Paraguay, and on Caribbean islands. The genus is estimated to be very young with natural history characteristics that have contributed to confusing species circumscriptions. The unique relationship of plants in the group with their butterfly pollinators makes Psiguria an interesting and important genus in tropical ecosystems. Both molecular and morphological approaches were used to investigate the monophyly of Psiguria, to elucidate the number of species in the genus, to discover sister relationships, and to identify characteristics for delineating species. Toward that end, an intensive screening of 141 primer combinations in search of phylogenetically informative low-copy nuclear markers was conducted along with a molecular phylogenetic analysis and a complete taxonomic revision of Psiguria. From the screening study, three potentially phylogenetically informative low-copy nuclear markers were discovered for Psiguria , 11 were found to be potentially useful in rosids, and 32 in other angiosperms. DNA sequences for eight chloroplast intergenic spacers ( ndhF-rpL32, ndhC-trnV, rps16-trnQ, trnS-trnG, psbZ-trnM, psbM-trnD, rpoB-trnC , and psbE-petL), ITS, and the nuclear serine/threonine phosphatase intron were obtained for 70 samples of Psiguria plus 14 outgroups. Phylogenetic analyses support the monophyly of Psiguria and a sister relationship between P. umbrosa and P. warscewiczii. In the final chapter, two reviews on the genus are presented -- one encapsulating the nomenclatural history, and one summarizing 35 years of ecological and natural history studies. In addition, morphological characters were databased, descriptions were written, and maps of geographic distribution were produced for all species. Considering both molecular and morphological data, six species of Psiguria are defined. To distinguish those species missing identifiable morphological characters, a set of DNA barcodes was developed. At least four chloroplast regions are required to differentiate species (ndhC-trnV, rps16-trnQ, rpoB-trnC, and ndhF-rpL32). Because of the absence of many morphological characters, two taxonomic keys are presented -- one using male flowers, and the other using the set of DNA barcodes along with consistent leaf characteristics and geographic distribution.
机译：皮古里亚阿恩。是葫芦科新热带葡萄的一小类，生长在从墨西哥南部到巴拉圭以及加勒比海岛屿的湿润和干燥热带森林中。估计该属非常年轻，具有自然历史特征，这些特征导致混淆了物种限制。该群体中的植物与蝴蝶授粉媒介之间的独特关系使Psiguria成为热带生态系统中一个有趣且重要的属。分子和形态学方法均用于研究Psiguria的单性，阐明属中物种的数量，发现姐妹关系，并鉴定描述物种的特征。为此，对141个引物组合进行了深入筛选，以寻找系统信息丰富的低拷贝核标记，并进行了分子系统发育分析和对Psiguria的完整分类学修订。从筛选研究中，发现了3个潜在的系统发育信息丰富的低聚核糖核酸标记物，其中11个可能对虫体有用，32个在其他被子植物中有用。八个叶绿体基因间隔子（ndhF-rpL32，ndhC-trnV，rps16-trnQ，trnS-trnG，psbZ-trnM，psbM-trnD，rpoB-trnC和psbE-petL）的DNA序列，ITS和核丝氨酸/苏氨酸获得了70个Psiguria样品和14个外群的磷酸酶内含子。系统发育分析支持Psiguria的单一性，以及P. umbrosa和P. warscewiczii之间的姐妹关系。在最后一章中，提出了两种有关该属的评论-一种概述了命名法的历史，另一种概述了35年的生态和自然历史研究。此外，对形态特征进行了数据库记录，编写了描述，并绘制了所有物种的地理分布图。考虑到分子和形态学数据，定义了六种Psiguria。为了区分那些缺少可识别的形态特征的物种，开发了一套DNA条码。至少需要四个叶绿体区域来区分物种（ndhC-trnV，rps16-trnQ，rpoB-trnC和ndhF-rpL32）。由于缺少许多形态特征，因此提出了两个分类学关键点-一个使用雄花，另一个使用DNA条码集以及一致的叶片特征和地理分布。

著录项

  • 作者

    Steele, Pamela Roxanne.;

  • 作者单位

    The University of Texas at Austin.;

  • 授予单位 The University of Texas at Austin.;
  • 学科 Biology Botany.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 219 p.
  • 总页数 219
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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