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首页> 外文学位 >Loss of heterozygosity in Drosophila melanogaster related to exposure of anesthetic gases and mutations in offspring
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Loss of heterozygosity in Drosophila melanogaster related to exposure of anesthetic gases and mutations in offspring

机译：果蝇果蝇杂合性的丧失与麻醉气体的暴露和后代的突变有关

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摘要

Introduction: The potential genotoxicity from exposure to volatile anesthetic agents is a concern for both anesthesia providers and patients undergoing procedures in which these agents are used. The purpose of this study is to compare the degree of genotoxicity of desflurane, isoflurane, and sevoflurane by analyzing loss of heterozygosity (LOH) in the offspring of Drosophila melanogaster (DM) after exposure to 1.0 minimum alveolar concentration (MAC) of these agents.;Overview: Meta-analysis demonstrates a relationship between operating room personnel exposure to anesthetic gases and risk for spontaneous abortion. Repeated exposure to even trace amounts of anesthetic gases may result in genotoxicity. Bench and clinical research have reported conflicting results concerning genotoxicity, cytotoxicity and gene expression following volatile anesthetic exposure.;Methods: In this quantitative, experimental laboratory study, the SMART assay was used to determine changes in eye color in female offspring of Drosophila melanogaster that were exposed to 1.0 MAC of desflurane, isoflurane, or sevoflurane. Experimental groups were compared to control groups, which consisted of a no treatment group and a group exposed to EMS, a known teratogenic chemical used to induce DNA mutations for scientific research.;Results: When assessing LOH in female offspring as measured by white spots in a normally red eye, all 3 experiments exhibited more white spots (LOH) than controls in isoflurane-exposed flies. The desflurane-exposed flies had significantly more LOH in 2 of the 3 experiments, and the sevoflurane-exposed flies showed no significant difference compared with the control (unexposed) group in all experiments. In 2 out of 3 experiments, Drosophila exposed to sevoflurane or desflurane showed a sex-skew in the offspring population, with more females than males observed.;Conclusion: Upon comparing experimental groups in all 3 experiments, sevoflurane resulted in the lowest incidence of LOH present in Drosophila, as measured by the SMART assay, whereas isoflurane resulted in the highest incidence of LOH measured. In 2 out of 3 experiments, desflurane also produced a significant LOH.
机译：简介：暴露于挥发性麻醉剂中可能产生的遗传毒性对于麻醉提供者和正在接受使用这些麻醉剂的程序的患者而言都是一个问题。这项研究的目的是通过分析果蝇黑腹果蝇（DM）的这些物质暴露于1.0最低肺泡浓度（MAC）后的杂合度（LOH）损失来比较地氟醚，异氟烷和七氟醚的遗传毒性程度。概述：荟萃分析显示手术室人员接触麻醉气体与自然流产风险之间的关系。反复接触甚至微量的麻醉气体可能会导致遗传毒性。基准和临床研究报告了挥发性麻醉剂暴露后在遗传毒性，细胞毒性和基因表达方面相互矛盾的结果。方法：在这项定量的实验性实验室研究中，SMART分析用于确定果蝇雌性后代的眼睛颜色变化。暴露于1.0 MAC的地氟醚，异氟烷或七氟醚中。将实验组与对照组进行比较，对照组由无治疗组和暴露于EMS的组组成，EMS是用于诱导DNA突变的已知致畸化学物质，用于科学研究。作为正常的红眼，在暴露于异氟烷的果蝇中，所有3个实验均显示出比对照组更多的白点（LOH）。在3个实验中的2个中，以地氟醚接触的果蝇的LOH明显更高，并且在所有实验中，以七氟醚接触的果蝇与对照组（未暴露）相比，无显着差异。在三分之二的实验中，接触七氟醚或地氟醚的果蝇在后代种群中表现出性别偏斜，观察到的女性多于男性;结论：在所有三个实验中比较实验组时，七氟醚导致LOH发生率最低果蝇中存在的果蝇（通过SMART分析测定），而异氟烷导致测得的LOH发生率最高。在三分之二的实验中，地氟醚也产生了显着的LOH。

著录项

  • 作者

    Luck, Eva.;

  • 作者单位

    Webster University.;

  • 授予单位 Webster University.;
  • 学科 Medicine.;Genetics.
  • 学位 M.S.
  • 年度 2017
  • 页码 88 p.
  • 总页数 88
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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