采集了崇明岛及上海周边地区共14个点的72只白山羊样本,并参照山羊线粒体基因组序列(NC_005044)设计了2对引物(CF1/CF2,MA1/MA2),进行了覆盖白山羊线粒体细胞色素b基因(cytb)全长的PCR扩增和测序.结果表明:72只白山羊的线粒体细胞色素b基因长度为1 140 bp.系统进化树分析表明,72个样本的线粒体cytb基因主要分成2个大群体,其中32个样本属于波尔山羊群体(BOR),另外40个样本则属于长江三角洲白山羊群体(YRD),该40个样本的cytb序列已登录Genbank(KY348291-KY348330).研究发现:目前崇明岛上的白山羊群体已存在大量的外来山羊品种的杂交,但其中2个采样点的6个样本单独聚为1支,提示该6个样本可能是崇明岛本地白山羊群体;统计发现该6个样本与本研究中其他长江三角洲白山羊在线粒体cytb基因上存在8个SNP位点.因此,应当对该两个点上的崇明岛白山羊进行种源保护和进一步研究.%The 72 samples of white goats were collected from Chongrning Island and Shanghai surrounding areas and the PCR amplification and sequencing of their mitochondrial cytochrome b (cyt b) genes were made through designing two pairs of primers (CF1/CF2,MA1/MA2) according to the mitochondrial genome sequences (NC_005044) of Capra hircus.The results showed that the length of their mitochondrial cyt b genes is 1 140 bp.The phylogenetic analysis showed that the 72 samples' mitochondrial cyt b genes can be divided into 2 major populations,the 32 samples belong to the Boer goat population (BOR),and the rest are the Yangtze River Delta white goat population (YRD)whose cyt b sequences have been recorded in the Genbank (KY348291-KY348330).The research indicated that there are a large number of hybrids in the white goat population of Chongming Island,but 6 samples from 2 sampling sites of Chongrning Island are independently clustered together,meaning that the 6 samples may be a local white goat population of Chongming Island.The statistic analysis found that there are 8 SNP loci in the mitochondrial cyt b genes of both those 6 samples and the other YRD population.So Chongming white goats on the 2 sampling sites of Chongming Island should be protected and further investigated.
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