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首页> 中文期刊> 《江西农业大学学报》 >Cu2+ 胁迫对中华绒螯蟹毒性作用及免疫相关基因表达的影响

Cu2+ 胁迫对中华绒螯蟹毒性作用及免疫相关基因表达的影响

         
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摘要

以中华绒螯蟹(Eriocheir sinensis)为实验对象,运用毒理学方法和实时荧光定量PCR技术分析Cu2+胁迫对中华绒螯蟹的毒性作用及免疫相关基因表达的影响.结果发现,试验72 h,低浓度组(0.1 mg/L和对照组)中无明显死亡现象,而高浓度组(0.5 mg/L和1.0 mg/L)中全部死亡,说明高浓度Cu2+胁迫对中华绒螯蟹具有明显的毒性作用;进一步分析发现,Cu2+(浓度为0.1 mg/L)胁迫对中华绒螯蟹免疫相关基因脂多糖和β-1,3-葡聚糖结合蛋白(LGBP)、fube、Dorsal、酚氧化酶原激活酶(PPAF)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽-S-转移酶(GSf)、细胞粘附蛋白(PXN)表达也产生显著影响,LGBP 基因mRNA表达量在胁迫36 h时显著高于对照组(P<0.05);PPAF基因 mRNA表达量在胁迫12 h和48 h显著高于对照组(P<0.05);fube基因mRNA在胁迫48 h时显著高于对照组(P<0.05),而72 h时显著低于对照组(P < 0.05);Dorsal 基因mRNA表达量在胁迫48 h时显著高于对照组(P<0.05);GPx基因mRNA表达量在胁迫36 h显著高于对照组(P<0.05),而72 h时显著低于对照组(P<0.05);GSf基因 mRNA表达量在胁迫36 h和48 h显著高于对照组(P<0.05);PXN基因mRNA表达量在胁迫12 h时显著高于对照组(P<0.05),而72 h时显著低于对照组(P<0.05).结果表明,重金属Cu2+胁迫对中华绒螯蟹具有明显的毒性作用,且显著影响其免疫模式识别蛋白相关基因(LGBP)、免疫信号传导相关基因(Dorsal、fube)及免疫效应相关基因(PPAF、GPx、GSf、PXN)的表达.%The toxic effects of Cu2+stress on the Chinese mitten crab,Eriocheir Sinensis,were analyzed by using toxicology methods.The results showed that the crabs at the low Cu2+concentration group(0.1 mg/L)and the control group(0 mg/L)had no obvious death,and the crabs at the high Cu2+concentration group(0.5 mg/L and 1.0 mg/L)all died after stress for 72 hours,which indicated that high concentration Cu2+stress had obvi-ous toxic effect on Eriocheir Sinensis.On the basis of this results,the influence of the Cu2+(concentration of 0.1 mg/L)stress on the immune-related genes expression,including lipopolysaccharide and beta-1,3-glucan bind-ing protein(LGBP),Tube,Dorsal,proPO-activating factor(PPAF),glutathione peroxidase(GPx),glutathione-S-transferase(GST),peroxinectin(PXN)was analyzed the using the real-time fluorescent quantitative PCR technique.The data indicated that the LGBP mRNA expression was significantly higher than that of the control group(P<0.05)after stressed for 36 h;the PPAF mRNA expression was significantly higher than that of the control group(P<0.05)after stressed for 12 h and 48 h;the Tube mRNA was significantly higher than that of the control group(P<0.05)after stressed for 48 h,and was significantly lower than that of the control group(P<0.05)after stressed for 72 h;the Dorsal mRNA expression was significantly higher than that of the control group(P<0.05)after stressed for 48 h;the GPx mRNA expression was significantly higher than that of the con-trol group(P<0.05)after stressed for 36 h,and was significantly lower than that of the control group(P<0.05) after stressed for 72 h;the GST mRNA expression was significantly higher than that of the control group (P<0.05)after stressed for 36 h and 48 h;the PXN mRNA expression was significantly higher than that of the control group(P<0.05)after stressed for 12 h,and was significantly lower than that of the control group (P<0.05)after stressed for 72 h.These results show that the Cu2+stress on Eriocheir Sinensis had significant impact on its immune pattern recognition protein genes(LGBP),immune signal transduction related genes (Dorsal,fube)and immune effect related genes(PPAF,GPx,GST,PXN).From what has been discussed a-bove,it can be concluded that heavy metal Cu2+stress has observable toxic effects and has significant impact on the immune related gene expression in the Chinese mitten crab,Eriocheir sinensis.

著录项

  • 来源
    《江西农业大学学报》 |2018年第2期|358-364|共7页
  • 作者

    毛振方; 林奇辰; 王彬宇; 张翠真; 陈志和; 简少卿; 赵大显;

  • 作者单位

    南昌大学 生命科学学院;

    江西 南昌330031;

    江西省水产动物资源与利用重点实验室;

    江西 南昌330031;

    南昌大学 生命科学学院;

    江西 南昌330031;

    江西省水产动物资源与利用重点实验室;

    江西 南昌330031;

    南昌大学 生命科学学院;

    江西 南昌330031;

    南昌大学 生命科学学院;

    江西 南昌330031;

    南昌大学 生命科学学院;

    江西 南昌330031;

    南昌大学 生命科学学院;

    江西 南昌330031;

    江西省水产动物资源与利用重点实验室;

    江西 南昌330031;

    南昌大学 生命科学学院;

    江西 南昌330031;

    江西省水产动物资源与利用重点实验室;

    江西 南昌330031;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 S968.2511;
  • 关键词

    中华绒螯蟹; Cu2+; 胁迫; 免疫相关基因; 毒性作用;

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