通过分析克隆获得的辣椒Rab11全长cDNA及其编码的氨基酸序列结构特征,为进一步研究辣椒Rab11功能奠定基础.通过对辣椒均一化cDNA文库的筛选分离获得了一个与葡萄Rab11小G蛋白VvRabAlf高度同源的全长cDNA,命名为CaRab11.序列分析结果表明:该cDNA包含有1164 bp的完整开放阅读框,编码217个氨基酸.该蛋白含有Rab GTP酶超家族保守的RabSF模体；Rab亚家族蛋白所特有的五个氨基酸序列RabF模体(RabF1-RabF5)；参与GTP/Mg++结合及GTP水解的G结构域(G1-G5:GDSGVGKS,T,DTAGQE,GNKADL及ETSAL)；两个构象变构域(Switch Ⅰ和SwitchⅡ)及与异戊二烯化相关的CCX序列.氨基酸同源性及进化分析同样表明CaRab11为辣椒小G蛋白Rab11家族新成员.%The characteristics of Rabll cDNA and deduced amino acids sequence in pepper were analyzed, which would lay the foundation for further investigation of the function of Rabll protein. One 1 164 bp full - length cDNA clone was isolated from a pepper normalized cDNA library, which encodes a putative protein composed of 217 amino acids. The full-length cDNA was named CaRabW. The amino acid sequence deduced by CaRabW cDNA showed high similarity to VvRabAlf protein from Vitis vinifera. CaRabll protein included conserved domains specific to Rab GTPase superfamily ( RabSF) , Rab subfamily specific regions (RabF, RabFl - RabF5 ) , five highly conserved GTP - binding consensus sequences ( GDSGVGKS, T, DTAGQE, GNKADL, and ETSAL) involved in GTP/ Mg + + binding and GDP hydrolysis, two conformation-al switch (Switch I and II) regions as well as C-terminal cysteines motif important to prenylation. Amino acids similarity and phylogenetic analysis also indicated that CaRabl 1 is a new member of small GTP - binding protein Rabll superfamily.
展开▼
