A detection method for Swine Hepatitis E Virus (SHEV) with RT- PCR technique was established as a reliable technological means for epidemiological investigation and clinical diagnosis of SHEV. Two pairs of primers were designed and synthesized according to SHEV' s genome sequence. The SHEV strain iso lated from Jiangxi Province, SHEV - JX - 1, was taken as templates. The RT - PCR detection method for SHEV was finally established with optimal reaction conditions. 230 bp fragment amplified from SHEV -JX-1 through the above method was part of SHEV Ⅳ gene. It was all negative to the results of the optimal examination method on other pig viruses. The established RT - PCR technique could identify 2.5 × 10 -8μg RNA of SHEV. Applied to testing some pig bile and intestinal contents collected from several pig farms in Jiangxi Province, the positive rates were 10％ and 4％, respectively. The RT - PCR detection method established in this study has the characteristics of sensitivity, high specificity and good repeatability, may be used in molecuhr epidemiological investigation and rapid clinical diagnosis of SHEV.%建立猪戊型肝炎病毒(Swine Hepatitis E Virus,SHEV) RT-PCR检测方法,为SHEV的流行病学调查、临床诊断提供可靠的技术手段.根据SHEV的基因组序列,设计并合成2对引物,以SHEV江西分离株SHEV-JX-1为模板,对反应条件进行了优化,建立了诊断SHEV的RT-PCR方法.该方法可从SHEV-JX-1中扩增出230 bp的片段,测序结果表明为SHEV Ⅳ型基因片段;对猪的其它病毒进行检测结果均为阴性;能检测出2.5×10-8μg的SHEV RNA;对江西部分猪场采集的胆汁及肠容物进行检测,结果胆汁中的阳性率为10%,肠容物的阳性率为4%.实验建立的RT-PCR方法具有敏感、特异、重复性好的特点,可用于SHEV的分子流行病学调查和临床快速诊断.
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