对怀玉山高山马铃薯茎尖再生苗6种病毒(马铃薯卷叶病毒、马铃薯Y病毒、马铃薯X病毒、马铃薯S病毒、马铃薯M病毒和马铃薯A病毒)进行了DAS-ELISA检测与分析,并比较不同茎尖脱毒方法的效率,旨在确定怀玉山高山马铃薯的病毒种类,并筛选出怀玉山高山马铃薯脱毒苗.结果表明,怀玉山高山马铃薯感染的病毒包括马铃薯卷叶病毒、马铃薯Y病毒、马铃薯X病毒、马铃薯S病毒、马铃薯M病毒和马铃薯A病毒6种."单纯的茎尖培养"以及"茎尖培养→热处理→茎尖培养"只能脱除部分病毒,而"茎尖培养→热处理→茎尖培养→热处理→茎尖培养"可脱除全部6种病毒.本试验成功获得了脱毒效果较好的编号为5-1-2和6-4-1以及脱毒效果最好的编号为5-3-2和6-2-2的怀玉山高山马铃薯茎尖再生脱毒苗.本试验结果为怀玉山高山马铃薯脱毒苗的工业化生产及其主粮化背景下高山马铃薯的产业化发展提供了技术支撑和理论依据.%In order to determine the virus species and screen out its virus-free plantlets , 6 viruses ( potato leafroll vi-rus, potato virus Y, potato virus X, potato virus S, potato virus M and potato virus A ) in plantlets regenerated from shoot-tips of Huaiyushan high mountain potato were detected and analyzed by DAS-ELISA, and the efficiency of dif-ferent shoot tip detoxification methods were compared .The results showed that there were 6 viruses including potato leafroll virus , potato virus Y , potato virus X , potato virus S , potato virus M and potato virus A in Huaiyushan high mountain potato .Two methods such as "simple shoot tip culture" and"shoot tip culture→thermotherapy→shoot tip culture" can only remove parts of the viruses , while "shoot tip culture→thermotherapy→shoot tip culture→thermo-therapy→shoot tip culture" could remove all 6 viruses.In this study, we successfully obtained the virus-free plant-lets of Huaiyushan high mountain potato , of which No.5-1-2 and No.6-4-1 had better virus-free effect and No.5-3-2 and No.6-2-2 had the best virus-free effect.The results will help to provide technical support and theoretical basis for virus-free plantlet industrial production of Huaiyushan high mountain potato and its industrialization development under the background of the fourth major staple food .
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