该研究以陕西汉中、河南林州、河北涉县和北京中国科学院植物研究所4个种源黄连木(Pistacia chinensisBunge)的苗木为对象,用分光光度计和色差仪对其叶绿素、类胡萝卜素、花色素苷含量及叶色参数(L*、a*、b*)进行了测定分析,探讨不同种源苗木秋季叶色变化规律及差异,揭示黄连木叶色呈现与叶片色素含量之间的内在关联,为筛选适合城市绿化的优良黄连木种源提供依据.结果表明:(1)在秋季叶片转色期,随着时间的推移,4个种源黄连木叶片的叶绿素、类胡萝卜素和花色素苷含量的比例呈现不同的变化趋势,其中:河北种源的花色素苷含量较高,叶片呈现红色;陕西种源叶绿素含量较高,叶片呈现绿色的时间较长;河南、北京种源处于两者之间.(2)各个种源黄连木的叶色参数a*值(红/绿)均与花色素苷含量呈正相关关系,与叶绿素含量呈负相关关系,且相关系数均达到显著水平(P＜0.05),各个种源叶色参数L*值(光泽明亮度)也与叶绿素含量间表现出显著或极显著的正相关性.研究发现,河北种源黄连木秋季的叶色最红,陕西种源黄连木叶片呈现绿色的时间最长;色差仪的应用实现了叶色和各色素含量间量化的关系.%In this study,four provenances of seedlings from Hanzhong of Shaanxi,Linzhou of Henan,Shexian of Hebei and Institute of Botany of Chinese Academy of Sciences of Beijing were selected as the research materials.This research analyzed the contents of chlorophyll,carotenoid,anthocyanin and leaf color parameters (L*,a*,b*) using spectrophotometer and colorimeter,explored the leaf color change in autumn and differences among provenances of Pistacia chinensis Bunge and discovered the internal relation between leaf color presentation and pigment content to select superior provenances of P.chinensis for the afforestation of our city.Our results showed that:(1) the chlorophyll,carotenoid and anthocyanin contents of leaves of P.chinensis in the four provenances showed different trend along with time in autumn.The content of anthocyanin in Hebei provenances was higher than others resulted in red leaves.However,green leaves in Shaanxi occurred longer than other provenances due to the higher chlorophyll content.The leaf color in Henan and Beijing provenances were between green and red.(2) For all four provenances of P.chinensis,there was a significant positive correlation between a* value(Red/Green) of leaf color parameters and anthocyanin content (P＜0.05),while a* value was negatively correlated with the chlorophyll content (P＜0.05).In addition,the L* value(Gloss brightness) of leaf color parameters showed significant or extremely significant positive correlation with chlorophyll content.Therefore,the leaf color of P.chinensis in Hebei provenances was the reddest in autumn,whereas green leaves in Shaanxi occurred the longest.The colorimeter makes it possible to quantify the relationship between leaf color and pigment contents.
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