The inhibition effect of antimicrobial peptides from the housefly ( Musca domestica) larvae on primary cell FBL-3 was tested under laboratory.Antibacterial peptides extracts was purified with C18 solid phase methods from induced housefly larva.Erythroleukemia mice model was prepared by injected 2.25 ×106/mL FBL-3 cells into C57BL/6 mouse via the tail vein.The primary culture of FBL-3 cells was harvested from the mice and utilized to test the inhibition effect of the antibacterial peptide extracts.When observed with trypan blue staining methods, antibacterial peptide mixture at a concentration of 250-350 μg/mL affects FBL-3 primary cells significantly with increased cell debris and more mortality rate.Cell apoptosis was also shown in the concentration range tested with CCK-8 kit.The reference concentration of antibacterial peptide would be applied in further tests in vivo.%本文初步探究不同浓度家蝇幼虫抗菌肽对FBL－3红白血病原代细胞增殖的影响。采用诱导、研磨、离心、 C18固相萃取等方法制备家蝇幼虫抗菌肽混合物，然后处理传代期的FBL－3细胞，调整浓度为2．25×106／mL并通过尾静脉注射C57BL／6小鼠，建立红白血病动物模型。待肿瘤生长较大时，处死并剥离肿瘤，再通过研磨、裂红液裂解、获取原代肿瘤细胞并培养。将抗菌肽混合物调整为不同浓度作用于传代期的FBL－3原代细胞，之后经倒置显微镜观察照相，台盼蓝染色试验计算死亡率、 CCK－8试剂盒做细胞毒性检测。结果显示抗菌肽混合物浓度为250～350μg／mL作用FBL－3原代细胞时，细胞碎片大量增多，死亡率增高，细胞毒性变大。初步证明了抗菌肽混合物在250～350μg／mL抑制细胞增殖效果明显，在300μg／mL浓度下抑制作用最强，本研究结果可作为动物模型体内实验的参考。
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