Objective To explore the value of Epstein-barr virus(EBV) antibody detection in diagnosis of nasopharyngeal carcinoma (NPC ) and infectious mononucleosis (IM). Methods ELISA and CLIA were used to evaluate the EBV 5 antibody in 535 hospitalized and outpatients. The results of the combined detection of EBV virus were analyzed by clinical pathological diagnosis. Results There was no statistical difference between the results of antibody tests in NPC and IM. The positive pattern of EB antibody spectrum in NPC was the highest in EBV-IgA, EBNA-IgG, EBVCA-IgG, EA-IgG mode, accounting for 84. 48％ of the positive rate, and IM was relatively more in EBVCA-IgG, EBVCA-IgM, EA-IgG mode, accounting for 54. 16％ of the total positive rate. The sensitivity of EB-NA-IgG and EBVCA-IgG in diagnosis of NPC was 100. 0％, and the specificity was 11. 3％ and 8. 5％ respectively. The specificity of EBVCA-IgM to NPC was as high as 95. 8％, while the sensitivity was only 1. 7％. In the IM, the sensitivity, specificity, accuracy and negative predictive value of the EBVCA-IgM single test were more than 90. 0％. The specificity and accuracy of EA-IgG reached 94. 5％ and 93. 1％, and the sensitivity was 70. 8％. Compared with the positive rate, the EBV-IgA and EA-IgG in NPC group were statistically significant(P 0.05). NPC EB病毒抗体谱阳性模式以EBV-IgA、EBNA-IgG、EBVCA-IgG、EA-IgG阳性数最多,占总阳性率的 84. 48％;传染性单核细胞增多症以EBVCA-IgG、EBVCA-IgM,EA-IgG阳性数相对较多,占总阳性率的54. 16％.EBNA-IgG和EBVCA-IgG诊断NPC的灵敏度均为100. 0％ ,特异度分别为11. 3％和8. 5％.EBVCA-IgM对NPC的特异度高达95. 8％ ,灵敏度低仅为1. 7％.传染性单核细胞增多症中的EBVCA-IgM单项检测的灵敏度、特异度、准确度和阴性预测值均达到90. 0％以上,EA-IgG特异度和准确度达到 94. 5％和93. 1％,灵敏度为70.8％.阳性率比较可看出 NPC组中EBV-IgA、EA-IgG明显高于成人其他疾病组,差异有统计学意义(P<0.05),传染性单核细胞增多症组中EB-VCA-IgM、EA-IgG明显高于儿童其他疾病组,差异有统计学意义(P<0.05).结论 EBNA-IgG、EBVCA-IgG在成人疾病中诊断的阳性率明显高于儿童,EBVCA-IgM阳性率在儿童传染性单核细胞增多症中有明显优势,高于成人疾病的诊断以及儿童其他疾病的诊断.
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