Objective To investigate the changes of the excitability of pyramidal neurons in the piriform cortex in-fluenced by enriched environment ( EE) . Methods Thirty C57 BL/6 mice of 15 day age were divided into 2 groups as follows: fifteen mice were reared in EE for 3 weeks ( EE group) and the others were reared in standard environment ( SE) for 3 weeks ( SE group) randomly. Current-clamp recordings with whole-cell patch clamp were made in pyramidal neurons of the piriform cortex. The excitability indexes of pyramidal neurons include inter-spike intervals ( ISI) , energy barrier ( Vts-Vr) and absolute refractory periods ( ARP) . Results The pyramidal neurons of the piriform cortex had lower Vts-Vr ( P＜0.05) and shorter ARP in EE group compared with SE group ( P＜0.05). Meanwhile, the ISI values significantly decreased in EE group compared with SE group (P＜0.05). Con-clusion Rearing in EE can enhance the pyramidal neuron sensitivity of the piriform cortex which indicates en-riched environment may help to accelerate pyramidal neurons development and maturate.%目的 探讨丰富环境干预下的小鼠梨状皮层锥体神经元兴奋性的变化特点.方法 选用15日龄的C57BL/6小鼠30只,随机分为丰富环境组( EE组)15只和常规环境组(SE组)15只,EE组采用丰富环境刺激饲养3周,SE组则在正常实验室环境下饲养3周.全细胞电流钳模式下记录小鼠梨状皮层锥体神经元的各电生理指标,锥体神经元的兴奋性指标包括动作电位间距( ISI)、能障( Vts-Vr)和绝对不应期(ARP).结果 与常规环境组比较,丰富环境干预下的小鼠梨状皮层神经元的ISI减小(P＜0. 05),Vts-Vr降低(P＜0.05),ARP缩短(P＜0.05).结论 丰富环境干预可提高梨状皮层锥体神经元的兴奋性,提示丰富环境可能有助于梨状皮层锥体神经元的发育成熟.
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